Eppendorf Centrifuge 5427 R User Manual Page 30
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Operation
Centrifuge 5427 R
English (EN)
30
5.4.6 Closing the rotor lid
1. Only QuickLock rotor lid: check that the external sealing ring correctly fits in the groove.
2. Vertically fit the rotor lid on the rotor.
3. Turn the rotor lid screw clockwise to seal the rotor.
Only QuickLock rotor lid:
Turn the rotor lid screw clockwise as far as it will go, until an audible "click" can be heard. The rotor is
not properly closed until an audible click is heard!
5.4.7 Closing the centrifuge lid
1. Check the correct attachment of the rotor and rotor lid.
2. Press the centrifuge lid down until it is gripped by the lid latch.
The lid will be automatically closed and the locking mechanism can be clearly heard.
The open key lights up blue. The display shows the symbol.
5.5 Cooling
The centrifuge cools or maintains the set temperature is the following requirements are met:
• The centrifuge is switched on.
• The centrifuge lid is closed.
• Only with continuous cooling: The set temperature is lower than the ambient temperature.
5.5.1 Set the temperature
1. To set the temperature, use the temp arrow keys to select a temperature between -11 °C and 40 °C.
2. Set the run time and g-force (rcf) or speed (rpm). Press the start/stop key to start the centrifugation.
The temperature can be changed during centrifugation.
5.5.2 Temperature display
WARNING! Risk of injury when opening or closing the centrifuge lid.
There is a risk of crushing your fingers when opening or closing the centrifuge lid.
When opening or closing the centrifuge lid, do not reach between the lid and device or into
the latching mechanism of the lid.
Always open the centrifuge lid completely to prevent it from falling.
If the rotor stops (continuous cooling), cooling is slower than during centrifugation or a
temperature control run.
Temperature display if the rotor stops Set temperature
Temperature display during centrifugation Actual temperature
- Centrifuge 5427 R 1
- Table of contents 3
- 1 Operating instructions 5
- 1.1 Using this manual 5
- 1.3 Symbols used 5
- 1.4 Abbreviations used 6
- 2 Product description 7
- 2.1 Main illustration 7
- 2.2 Delivery package 8
- 2.3 Features 8
- 2.4 Rotors 9
- 2.4.2 Rotor FA-45-24-11 10
- 2.4.3 Rotor FA-45-24-11-Kit 11
- 2.4.6 Rotor F-45-48-5-PCR 14
- 2.4.7 Rotor S-24-11-AT 14
- 3.1 Intended use 15
- 3.2 User profile 15
- 3.3 Application limits 15
- 3.5 Warnings for intended use 17
- 4 Installation 21
- 4.1 Selecting the location 21
- 4.2 Preparing installation 21
- 4.3 Installing the instrument 22
- 5 Operation 23
- 5.2 Menu navigation 25
- 5.3 Menu 25
- 5.4.2 Inserting the rotor 27
- 5.4.4 Replacing the rotor 27
- 5.4.5 Loading the rotor 28
- 5.5 Cooling 30
- 5.5.3 Temperature monitoring 31
- 5.5.4 Start FastTemp 31
- 5.5.5 Continuous cooling 32
- 5.6 Centrifuging 33
- 5.7 Standby mode 35
- Operation 36
- English (EN) 36
- 6 Maintenance 37
- 6.2 Cleaning/disinfection 37
- 6.4 Glass breakage 39
- 6.5 Replacing fuses 40
- 7 Troubleshooting 41
- 7.1 General errors 41
- 7.2 Error messages 42
- 7.3 Emergency release 44
- 8.1 Transport 45
- 8.2 Storage 45
- 8.3 Disposal 45
- 9 Technical data 47
- 9.1 Power supply 47
- 9.2 Ambient conditions 47
- 9.3 Weight/dimensions 47
- 9.4 Application parameters 48
- (according to DIN 58 970) 49
- Technical data 50
- 10 Ordering Information 51
- 10.1 Centrifuge 5427 R 51
- 10.2 Accessories 51
- 10.2.2 Adapters 52
- 10.3 Safety 53
- Ordering Information 54
- Italiano (IT) 58
- 2 Descrizione del prodotto 59
- 2.1 Illustrazione generale 59
- Descrizione del prodotto 60
- 3.1 Uso conforme 61
- 3.2 Richiesta all'utente 61
- 3.3 Limiti di applicazione 61
- 3.5.3 Uso errato dei rotori 65
- 4 Installazione 69
- 6791010 8 71
- 5.2 Navigazione del menu 73
- 5.4.2 Inserimento del rotore 75
- 5.4.4 Cambio del rotore 75
- 5.4.5 Caricamento del rotore 76
- 5.5 Raffreddamento 78
- 5.6 Centrifugazione 81
- 5.6.2 Centrifugazione breve 82
- 5.6.4 Dopo la centrifugazione 83
- 6 Manutenzione 85
- 6.4 Rottura di parti in vetro 88
- 6.5 Sostituzione dei fusibili 89
- Manutenzione 90
- 7 Risoluzione dei problemi 91
- 7.1 Anomalie generiche 91
- 7.2 Messaggi di anomalia 92
- 7.3 Sblocco di emergenza 94
- 8.1 Trasporto 95
- 8.2 Immagazzinamento 95
- 8.3 Smaltimento 95
- Evaluate your manual 106
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