Eppendorf Centrifuge 5427 R User Manual download pdf (Page 28)

Operation

Centrifuge 5427 R

English (EN)

5.4.5 Loading the rotor

5.4.5.1 Loading a fixed-angle rotor

To load the rotor, proceed as follows:

1. Check the maximum payload (adapter, tube and contents) for each rotor bore.

The information about this can be found on every rotor and in this operating manual (see Rotors on p. 9).

2. Load rotors and adapters only with the tubes intended for them.

3. Insert sets of two tubes in the opposite bores of the rotor. To ensure symmetric loading, tubes that are

arranged opposite each other must be of the same type and contain the same filling quantity.

To minimize weight differences between filled sample tubes, we recommend taring with a scale. This

will reduce wear on the drive and reduce running noise.

CAUTION! Risk of injury due to asymmetric loading of a rotor.

 Load rotors symmetrically with identical tubes.

 Only load adapters with suitable tubes.

 Always use the same type of tubes (weight, material/density and volume).

 Check symmetric loading by balancing the adapters and tubes used with scales.

CAUTION! Risk from damaged or overloaded tubes.

 When loading the rotor, observe the safety precautions on dangers as a result of

overloaded or damaged tubes (see Warnings for intended use on p. 17).

Use matching rotor lids

• Fixed-angle rotors may only be operated with the appropriate rotor lid in each case. The

rotor name on the rotor must be the same as the name on the rotor lid.

• To carry out an aerosol-tight centrifugation, an aerosol-tight rotor (label: red ring) and the

corresponding aerosol-tight rotor lid (label: aerosol-tight and red lid screw) must be used.

The device automatically detects imbalances during operation and stops the run immediately

with an error message and a signal tone.

 Check the load, balance the tubes and restart the run.

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Eppendorf Centrifuge 5427 R User Manual Page 28