Eppendorf Centrifuge 5427 R User Manual download pdf (Page 27)

Operation

Centrifuge 5427 R

English (EN)

5.4 Preparing for centrifugation

5.4.1 Switching on the centrifuge

 Switch the centrifuge on using the mains/power switch.

The centrifuge lid opens automatically after switching on using the mains/power switch.

The parameter settings of the last run are displayed.

5.4.2 Inserting the rotor

1. Fit the rotor vertically on the motor shaft.

2. Insert the supplied rotor key into the rotor nut.

3. Turn rotor key clockwise until the rotor nut is firmly tightened.

5.4.3 Automatic rotor detection

 Manually turn the rotor counterclockwise to trigger rotor detection.

• The name of the rotor appears in the display.

• The g-force (rcf) and speed (rpm) are automatically limited to the maximum permissible value for the

rotor.

5.4.4 Replacing the rotor

1. Turn the rotor nut counterclockwise using the supplied rotor key.

2. Remove the rotor by lifting it vertically.

3. Vertically place another rotor on the motor shaft.

4. Insert the supplied rotor key into the rotor nut.

5. Turn rotor key clockwise until the rotor nut is firmly tightened.

6. In order to trigger the rotor detection, turn the rotor counterclockwise by hand.

• The name of the rotor appears in the display.

• The g-force (rcf) and speed (rpm) are automatically limited to the maximum permissible value for the

rotor.

The centrifuge has automatic rotor detection. It detects a newly inserted rotor and displays the

name of the rotor for 2 s. g-force (rcf) and speed (rpm) are automatically limited to the

maximum permitted value for the rotor.

Rotor detection can also be triggered by short spin centrifugation:

 Press the short key until the name of the rotor appears in the display.

Error message after rotor change

• If you start centrifuging immediately after a rotor change, the centrifuge has not carried

out an automatic rotor detection yet. The speed set for the previous rotor may exceed the

maximum permitted speed for the new rotor. In this case, the centrifuge stops after the

automatic rotor detection and displays SPEED. The new maximum permitted speed

appears in the display. You can then restart the centrifuging with this setting or adjust the

speed as necessary.

 After each rotor change, check whether the new rotor is detected by the device. Check the

set g-force (rcf) or speed (rpm) and adjust it if necessary.

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Eppendorf Centrifuge 5427 R User Manual Page 27