Eppendorf 5430 R Centrifuge User Manual download pdf (Page 34)

Operation

Centrifuge 5430/5430 R

English (EN)

5.4.5.2 Swing-bucket rotor

Prerequisites

• A combination of rotor, carrier and adapter, approved by Eppendorf.

• Matching and tested tubes and plates.

To load the rotor, proceed as follows:

1. Check the carrier grooves for cleanliness and grease lightly with pivot grease 

(order no. Int.: 5810 350.050 / North America: 022634330).

Dirty grooves and pivots prevent carriers from swinging out evenly.

2. Hang the buckets into the rotor.

All rotor positions must be loaded with carriers.

3. Check that all carriers are hanging properly and can swing freely.

4. Carry out a manual loading and swing test the first time a tube or plate type is used.

5. Check the maximum load per carrier (adapter, tube or plate and contents) and the loading height.

The information about this can be found on the rotor and in this operating manual (see Rotors on p. 12).

Spin columns

For centrifuging spin columns in the rotor FA-45-24-11-Kit, you can leave the tube lids open.

However, this can only be done using the tubes provided by kit manufacturers for this

purpose. For reliable centrifugation, you must lean the open tube lids against the edge of the

rotor. Ensure that this does not involve the lids projecting beyond the edge of the rotor, and

then put on the associated rotor lid.

NOTICE! Filling the plates too high can cause overflowing.

During the run the meniscuses in the tubes along the edges of the plates are at an angle. This

is due to the centrifugal forces and cannot be avoided.

 Fill the wells of the plates to a maximum of 2/3 of the max. capacity.

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Eppendorf 5430 R Centrifuge User Manual Page 34