Eppendorf InjectMan NI 2 User Manual

InjectMan® NI 2 Operating ManualT_R_InjectMan_NI2.fm Seite 1 Donnerstag, 6. Dezember 2012 6:03 18

63On the A module is the tool mount for the probe holders. Tools with a diameter, or width, of 4 mm and weighing max. 100 g can be inserted, e.g. cap

64 3.3.1 Tool movement Moving the dynamic joysticks moves the capillaries in one plane. The speed of the motion is determined by the speed of the de

65 Axial Activate or disable axial movement. Pos 1 / Pos 2 Approach a stored position.Store current position by pressing and holding down the key.Cle

66 Turn wheel Set the speed in the current speed mode. Display of selected speed on-screen. 3.3.3 Display In normal operation the display shows the

67– The packaging and the transport guards should be kept for use if the device is transported again later! – The control board must not be carried o

68 4.2.1 Components of the module unit Fig. 3: Module unit, assembled (here right side mounted)1 Motor unit for horizontal movements (X/Y module)

69The hole spacing is 5°. By moving the mounting point of the angle guide 5° upwards, the adjustable angle changes each time to 50° to 30°. By moving

70 4.2.4 Angle adjuster Set the desired angle. Mounting the angle guide at the centre hole and adjusting the A module to 0° corresponds to an angle

71For right side mounting, the hinge must be at the right outer (not visible here, on the rear).The module unit is mounted from above onto the hinged

72The hinged bracket is opened up and joined to the motor module by its inner inclined surface by means of the M5x16 screw. Close the hinged bracket.

2Bedienungsanleitung. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3Operating Ma

73 4.3.1 Right and left side mounting The module unit mounting is dependent on the chosen side of the microscope.The module unit can be mounted on t

74According to the mounting mode on the left side of the adapter. Note : In the "Install" menu (section 5.4, subsection 5.4.2.6) the parame

75To avoid having to reposition every time a capillary is changed, the positioning aid can be mounted on the universal capillary holder so it fits in

76 4.4.1 Transmission cable 5181/5246 For optimum use of the INJECT function (see section 5.1), the InjectMan ® NI 2 micromanipulator and the micro

77Section 5.1 provides a brief summary of how the commands can be used for the injection procedure. Section 5.2 provides additional information on th

78The new speed is shown on the display as a modified block diagram.If the capillary touches the medium surface for example (visually monitored), swit

79 Changing the limit As a result of moving the petri dish, the limit may need to be adjusted. This is done using the arrow keys to the left and righ

80– Carefully slacken knurled screw of tool mount and positioning aid a little way. Carefully bring the capillary holder with the capillary into the

81Or the capillary is brought into the focal plane like the cell. – Press the "Limit" key.The defined limit is indicated on the display.To c

82 5.2.3 Injection Pressing the joystick button activates the Inject function. The capillary moves automatically from its current position, the sear

561 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 582 Intended use

83– Synchronisation Move: No pressure build-up occurs. If the joystick button is held down the capillary stops as shown in Fig. 6.3 and a separate in

84 5.2.5 Field of movement To utilise the benefits of axial injection to the full, only one motor (A module) is used. This permits less harmful injec

85The keypad can be used to make settings or initiate movements directly. Frequently used settings can be entered by means of key combinations. The L

86 5.3.2 Setting the speed The speed can be set in three ways:Directly using the turn wheel, by pressing and holding down the key, or by way of the

87 5.3.3 Home The tools moves axially out of the working radius.The function is intended for changing capillaries, or for quick removal of capillari

88 5.3.5 Pos 1 / Pos 2Approach a stored position. Store current position by pressing and holding down the key (acoustic acknowledgement). Clear

895.3.8 MenuToggles between menu and back. Parameters can be selected or changed in the menu using the joystick. For a detailed description see secti

905.3.11 Injection speedBy pressing and holding down the "Limit" key the injection speed can be adjusted. The current selected speed is ind

91Moving joystick to the right moves the cursor into the submenu. The left hand column shows the possible parameters, the right hand column the associ

92To switch to other main menu options press the joystick button again or move the joystick to the left.Alternatively, the or keycan be used.To swi

575.4 Parameter settings via menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 935.4.1 Meanings of param

935.4.1 Meanings of parameters in menu5.4.1.1 Main menu optionsSpeed: Includes all speed parameters.Inject: Includes all the parameters relevant t

945.4.2.2 Inject InjSpeed: Speed of (axial) injection movement. Adjustable from 7,500 to 5 µm/s in 5-µm/s increments.Synchron: Synchronisation of co

955.4.2.3 StepInjThis special parameter enables the tool to be move forward automatically from its current position by a specific distance (D) at a sp

965.4.2.5 PositionPosSpeed: Sets the speed at which the stored position is approached.Adjustable from 7,500 to 5 µm/s in 5-µm/s increments.ClearAll:

975.4.2.7 FunctionZeroCord: This function sets the coordinates of the X, Y and Z axes to zero. CentrMot: This function sets the motors to a mid pos

985.5.2 Software version pollingAs an additional software version polling facility (also visible during the power-up routine on the display) the vers

99Before cleaning the InjectMan ® NI 2, disconnect the plug.Ensure that no fluids enter into the inside of the micromanipulator in order to avoid sho

100The following error situations are conceivable: Remedy – Motor axes move in wrong direction or do not match joystick movement.– Check installation

101The following warnings or error messages may be displayed: Remedy – Warning 001 Check Injector and cable: The injector is not ready or there is no

102– Error 044 – 046In self-test wrong values defined for displayed axis.– Repeat self-test, reset, general reset. If error recurs: Service– Error 06

58The Eppendorf InjectMan ® NI 2 micromanipulator is specially designed as a micromanipulator for use in microinjection. Its optimum functionality a

103 Power supply Input: 120 – 240 V AC; 50 – 60 Hz; < 50 WOutput: 15 – 18 V DC; 25 W Control board 15 – 18 V DC; 25 WContamination level: II O

104 Order no. InjectMan ® NI 2, Europe 5181 000.017InjectMan ® NI 2, USA 5181 000.025InjectMan ® NI 2, UK/Hongkong 5181 000.033InjectMan ® NI 2,

105 Accessories (non-standard) Order no. Universal capillary holder for accommodating Femtotips ® , Femtotips ® II or grip heads5176 190.002Grip he

106 Order no. FemtoJet ® expressProgrammable microinjector incl. connection for external pressure supplyVersion Europe 5248 000.017Version USA and C

107 Order no. InjectMan ® NI 2, USA 920000029InjectMan ® NI 2, international 5181 000.017InjectMan ® NI 2, UK/Hongkong 5181 000.033InjectMan ® N

108 Accessories (non-standard) Order no. Universal capillary holder for accommodating Femtotips ® , Femtotips ® II or grip heads920007392Grip head

109 Order no. FemtoJet ® expressProgrammable microinjector incl. connection for external pressure supplyVersion USA and Canada 5248 000.050Version E

110From the Install submenu up to 4 Users (users/applications) can be activated. The Users can be assigned dedicated parameters for speed, injection

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59The InjectMan NI 2 enables an electronically controlled axial injection movement. It is designed for controlled, gentle, semi-automatic microinject

Your local distributor: www.eppendorf.com/worldwideEppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 53801-0 · Fax: +49 40 538 01-556 · E-mail: epp

60 • Caution when mounting tools!When the tool is mounted, the motors are swung out forwards and the capillaries face the front. Handle with care! •

61Before checking that the device has been delivered complete and installing it, please read the following section thoroughly. • The control board mu

62The Eppendorf InjectMan ® NI 2 micromanipulator is made up of three components:– Control board– Module unit– Mains power supply unitFig. 1: Injec