Eppendorf InjectMan 4 User Manual

Register your instrument! www.eppendorf.com/myeppendorfInjectMan® 4Operating manual

Product descriptionInjectMan® 4English (EN)10The capillary is controlled by a joystick. A greater forwards or backwards movement of the joystick resul

Evaluate your manualGive us your feedback.www.eppendorf.com/manualfeedbackYour local distributor: www.eppendorf.com/contactEppendorf AG · 22331 Hambur

Product descriptionInjectMan® 4English (EN)112.3.1 Motor moduleThe motor module is made up of three modules. The capillary can be moved in all three s

Product descriptionInjectMan® 4English (EN)122.3.2 Control boardThe control board contains the key pad, the display and the joystick, and, to the side

Product descriptionInjectMan® 4English (EN)13Abb. 2-4:Control board - rearFig. 2-4: Control board - rear1 Mains power switch On/Off2 Connection for an

Product descriptionInjectMan® 4English (EN)142.4 Control panelWith the keys on the control panel you can switch on the control board and select the si

Product descriptionInjectMan® 4English (EN)152.5 JoystickThe joystick controls the capillary in all three spatial axes. The movement of the capillary

Product descriptionInjectMan® 4English (EN)162.5.2 Direction of movement of the joystickThe joystick can be moved along the horizontal plane. This con

Product descriptionInjectMan® 4English (EN)172.6 Speed rangesThere are three speed ranges in the movement range of the joystick. The speed can be set

SafetyInjectMan® 4English (EN)183Safety3.1 Intended useThe InjectMan 4 has been designed and manufactured for use in biological, chemical and physical

SafetyInjectMan® 4English (EN)193.3 Warning signs on the device3.4 Information on product liabilityIn the following cases, the designated protection o

5192 900.010-00/072013Copyright© 2013 Eppendorf AG, Hamburg.Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Ger

InstallationInjectMan® 4English (EN)204 Installation4.1 Preparing installation1. Check the packaging for damage.2. Carefully remove the motor module a

InstallationInjectMan® 4English (EN)214.3 Mounting overviewAbb. 4-1:Overview for right-hand s ide mountingFig. 4-1: Overview for right-hand side mount

InstallationInjectMan® 4English (EN)224.3.1 Module (X, Y, Z)Abb. 4-2:Y-moduleFig. 4-2: Y-module1 Cable2 Module identification3 Movable rail4 ScaleMove

InstallationInjectMan® 4English (EN)234.3.2 Z-module holderAbb. 4-3:Z-module holder, front and backFig. 4-3: Z-module holder, front and back4.3.3 Slid

InstallationInjectMan® 4English (EN)244.3.4 Angle headAbb. 4-5:Angle headFig. 4-5: Angle head1 IdentificationFor setting the angle2 Knurled screwFor s

InstallationInjectMan® 4English (EN)254.3.5 Swivel jointAbb. 4-6:Swivel joint for right side mountingFig. 4-6: Swivel joint for right side mounting4.

InstallationInjectMan® 4English (EN)264.4.1 Mounting the Z-modulePrerequisites• 3 mm Allen torque key4.4.2 Mounting the Y-modulePrerequisites• 3 mm Al

InstallationInjectMan® 4English (EN)274.4.3 Mounting the X-modulePrerequisites• 3 mm Allen torque key1. Push the YZ-connector onto the fixed rail of t

InstallationInjectMan® 4English (EN)284.4.4 Mounting the angle headPrerequisites• 3 mm Allen torque key4.5 Inserting the capillary holder into the ang

InstallationInjectMan® 4English (EN)294.6 Inserting the capillary into the capillary holder1. Loosen the knurled screw on the angle head.2. Insert the

Table of contentsInjectMan® 4English (EN)3Operating manualInjectMan® 4seeon p.Fig.Tab.p.English (EN)Operating manualTable of contents1 Operating instr

InstallationInjectMan® 4English (EN)304.6.1 Inserting Femtotips1. Remove the grip head.2. Screw the adapter for Femtotips into the universal capillary

InstallationInjectMan® 4English (EN)314.8 Aligning the motor moduleCentrally align the modules in order to be able to use the full movement range of t

InstallationInjectMan® 4English (EN)324.8.3 Aligning the width4.8.4 Aligning the angle head4.9 Entering mounting parametersTo facilitate remounting th

InstallationInjectMan® 4English (EN)334.9.2 Motor module4.9.3 Angle headPosition Description Position [mm]1 Z-module holder on the adapter2 Z-module o

InstallationInjectMan® 4English (EN)344.10 Converting the swivel joint for left side mountingPrerequisites• 2 mm Allen key• Right side mounting marks

InstallationInjectMan® 4English (EN)353. Remove the stop plate.4. Rotate the lower joint back.5. Rotate the upper joint by 180°.The sliders must be at

InstallationInjectMan® 4English (EN)366. Align the left side mounting marks (I).7. Fit the stop plate in such a way that the pins sit in the holes of

InstallationInjectMan® 4English (EN)374.11 Converting the angle head for left side mountingPrerequisites• 1.3 mm Allen key10.Check the position of the

InstallationInjectMan® 4English (EN)384.12 Connect the motor module to the control board2. Rotate the angle head by 180°.3. Push the knurled screw ont

InstallationInjectMan® 4English (EN)39Prerequisites• InjectMan 4 is switched off.• The power cable is disconnected.1. Connect the module (X,Y,Z) plug

Table of contentsInjectMan® 4English (EN)44.4 Mounting the motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 254.

InstallationInjectMan® 4English (EN)404.13.1 First set-up wizardPrerequisites• The micromanipulator is switched on.1. Select the First set-up applicat

InstallationInjectMan® 4English (EN)416. Select the Execute function to move all motors to the middle position.7. Select Next.8. Insert the capillary

InstallationInjectMan® 4English (EN)42• The installation is complete and the modules are adjusted.• The application screen displays My application17.S

InstallationInjectMan® 4English (EN)434.14 Connecting an external deviceThe following devices can be connected to the control board:• Eppendorf FemtoJ

InstallationInjectMan® 4English (EN)444.14.3 Connecting the ComputerPrerequisites• A data cable is available.• Devices are switched off.1. Connect the

SoftwareInjectMan® 4English (EN)455 Software5.1 DisplayThe display shows current settings, for example, the selected working range, the position of th

SoftwareInjectMan® 4English (EN)465.1.2 Display of coordinatesAbb. 5-2:Display of coordinatesFig. 5-2: Display of coordinates1 Motor limit stop+ = pos

SoftwareInjectMan® 4English (EN)475.1.3 Menu displayAbb. 5-3:Menu and parameter displayFig. 5-3: Menu and parameter display5.2 ApplicationsAbb. 5-4:Ap

SoftwareInjectMan® 4English (EN)48Application selection• Select the application• Store the main application5.2.1 Application parameter Predefined func

SoftwareInjectMan® 4English (EN)495.2.3 Application – ICSIThis application is suitable for intracytoplasmic sperm injection.Abb. 5-6:Application 2 ICS

Table of contentsInjectMan® 4English (EN)56 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

SoftwareInjectMan® 4English (EN)505.2.5 Application – PhysiologyThis application is suitable for physiology.Abb. 5-8:Application 4 Phys iologyFig. 5-8

SoftwareInjectMan® 4English (EN)515.3 Main menuAbb. 5-10:Main menuFig. 5-10: Main menuMenu ParameterSpeed Set the speed parametersInject Set the injec

SoftwareInjectMan® 4English (EN)525.4 Navigating the menuAbb. 5-11:Software navigationFig. 5-11: Software navigationNavigate the menu using the arrow

OperationInjectMan® 4English (EN)536Operation6.1 Switching the device on or off6.1.1 Switching on the device1. Switch on the device at the mains power

OperationInjectMan® 4English (EN)54• Current movements are stopped.• The motor module remains switched on so that the step motors keep their current p

OperationInjectMan® 4English (EN)551. Move the capillary out of the working range using the home key.2. Swing the X-module forwards.3. Undo the grip h

OperationInjectMan® 4English (EN)566.5 Changing the sample6.6 Changing the speed range 6.6.1 Changing parameters using the selection dialCAUTION! Risk

OperationInjectMan® 4English (EN)576.6.2 Changing parameters in the menuPrerequisites• An application has been selected.6.7 Capillary positionsTo secu

OperationInjectMan® 4English (EN)586.7.2 Moving to the position using the softkeyPrerequisites• At least one position is stored.1. Press a softkey wit

OperationInjectMan® 4English (EN)596.7.5 Overwriting a stored positionPrerequisites• A position is stored.1. Press the softkey for a different positio

Table of contentsInjectMan® 4English (EN)66.13 Home function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

OperationInjectMan® 4English (EN)606.8.2 Deleting the lower limit1. Press Z-axis Limit.• The limit is deleted.6.8.3 Defining the upper limit1. In the

OperationInjectMan® 4English (EN)616.9 Horizontal limitFor the X-axis, a limit can be defined for a horizontal injection. This prevents the capillary

OperationInjectMan® 4English (EN)626.10 Speed functionIn the Speed menu you can set the speed of the working range and the speed at which certain posi

OperationInjectMan® 4English (EN)636.11 Inject functionThis function performs an automatic injection movement. You can set the injection speed, the in

OperationInjectMan® 4English (EN)646.11.2 Executing the Inject functionPrerequisites• A FemtoJet is connected.• A lower limit (Z-axis Limit) has been

OperationInjectMan® 4English (EN)656.12 Step injection functionWith this function you can execute a straight injection over a defined distance. Step i

OperationInjectMan® 4English (EN)666.12.2 Executing the Step injection functionPrerequisites• A FemtoJet is connected.• Step injection is assigned to

OperationInjectMan® 4English (EN)676.13.3 Move the capillary out with the home keyPrerequisites• The parameters in the Home menu have been defined.6.1

OperationInjectMan® 4English (EN)68• The value for the offset is displayed in the status field.6.13.6 Terminate the home function.6.14 Clean functionW

OperationInjectMan® 4English (EN)696.14.2 Setting the parameter for Clean1. Set Home speed.2. Set Clean distance.3. Close the menu.6.14.3 Execute the

Table of contentsInjectMan® 4English (EN)77 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

OperationInjectMan® 4English (EN)706.15 PiezoXpert function6.15.1 PiezoXpert menu and parametersAbb. 6-6:Menu – PiezoXpertFig. 6-6: Menu – PiezoXpertP

OperationInjectMan® 4English (EN)716.15.2 Execute the PiezoXpert functionPrerequisites• A PiezoXpert is connected.1. Activate Synchr. PiezoXp.2. Set P

OperationInjectMan® 4English (EN)726.16.4 Module parameters6.17 Function functionFunction allows you to reset the parameters and coordinates and to mo

OperationInjectMan® 4English (EN)736.17.2 Executing Zero coordin.1. Select the parameters and confirm with Enter.2. Confirm Execute with Enter.• All c

OperationInjectMan® 4English (EN)746.18 Softkeys functionWith this function you can assign programs to free softkeys. Assigned softkeys are indicated

OperationInjectMan® 4English (EN)756.18.2 Executing Softkeys1. Select the free softkey and confirm with Enter.2. Select the desired parameter and conf

OperationInjectMan® 4English (EN)766.18.3 Executing Joystick keyPrerequisitesThe My application application has been selected.1. Select Joystick key a

OperationInjectMan® 4English (EN)776.19.3 Defining the application as the start screen1. Select the application.2. Confirm with Enter.3. Close the men

OperationInjectMan® 4English (EN)786.20.2 Execute the Selftest function1. Select Selftest and confirm with Enter.2. Confirm Execute with Enter.The scr

TroubleshootingInjectMan® 4English (EN)797 Troubleshooting7.1 General errors7.1.1 Motor module7.1.2 Capillary7.1.3 Control board and displaySymptom/m

Operating instructionsInjectMan® 4English (EN)81 Operating instructions1.1 Using this manual Read this operating manual completely before using the d

TroubleshootingInjectMan® 4English (EN)807.1.4 Joystick7.1.5 Software and parameters7.2 Error messages7.2.1 WarningsThe display does not show anything

TroubleshootingInjectMan® 4English (EN)817.2.2 ErrorWARNING 5 • Z-module is not connected.  Switch off the device. Connect the plug of the Z-module

MaintenanceInjectMan® 4English (EN)828 Maintenance8.1 Replacing fusesThe fuse holder is located between the mains connection socket and the mains powe

MaintenanceInjectMan® 4English (EN)838.3 Service and maintenanceApplication-specific service and safety inspections are not requiredThe Eppendorf AG s

Technical dataInjectMan® 4English (EN)849 Technical dataMotor moduleTravel, maximum 20 mmStep motors X-module, Y-module, Z-moduleWeight 2150 gModule (

Technical dataInjectMan® 4English (EN)859.1 Power supply9.2 Interfaces9.3 Ambient conditionsControl boardMains connection 120 – 240 V AC, 50 – 60 HzPo

Transport, storage and disposalInjectMan® 4English (EN)8610 Transport, storage and disposal10.1 Storage10.2 Decontamination before shipmentIf you are

Transport, storage and disposalInjectMan® 4English (EN)8710.4 DisposalIn case the product is to be disposed of, the relevant legal regulations are to

Ordering InformationInjectMan® 4English (EN)8811 Ordering Information11.1 InjectMan 411.2 Accessories for InjectMan 4Order no. (International)Order no

Ordering InformationInjectMan® 4English (EN)8911.3 Tools for InjectMan 411.4 Microscope adapterOrder no. (International)Order no. (North America)Descr

Product descriptionInjectMan® 4English (EN)92 Product description2.1 Delivery package2.1.1 Tools2.1.2 Accessories2.2 FeaturesThe micromanipulator Inje

Ordering InformationInjectMan® 4English (EN)9011.5 Accessories for adapters for microscope11.6 Capillaries for research applicationsOrder no. (Interna

Ordering InformationInjectMan® 4English (EN)9111.7 CapillariesProducts are registered as a medical device in Europe (according to the Medical Device D

Ordering InformationInjectMan® 4English (EN)9211.9 CellTram and accessoriesProducts are registered as a medical device in Europe (according to the Med

Ordering InformationInjectMan® 4English (EN)9311.11 FemtoJet express11.12 Accessories for FemtoJet/FemtoJet expressOrder no. (International)Order no.

Ordering InformationInjectMan® 4English (EN)9411.13 PiezoXpertOrder no. (International)Order no. (North America)DescriptionEppendorf PiezoXpertfor pie

IndexInjectMan® 4English (EN)95IndexAAmbient conditions ... 85CCable...

IndexInjectMan® 4English (EN)96Service provisions ... 83Software navigation... 52Speed range ...