Eppendorf 5810 R Centrifuge User Manual

Centrifuge 5804/5804 R/5810/5810 ROperating manual 5820 900.040

Centrifuge 5804/5804 R/5810/5810 R — Operating manual10 1.4 Abbreviations usedMTP Micro test plateNN Mean sea level (MSL)PCR Polymerase chain reacti

11Centrifuge 5804/5804 R/5810/5810 R — Operating manual 2 Product description2 Product description2.1 Main illustrationAbb. 1: Depiction of Centrifu

Centrifuge 5804/5804 R/5810/5810 R — Operating manual12 Only 5804R and 5810R:2.3 FeaturesThe versatile 5804/5804R/5810/5810R centrifuge has a ca

13Centrifuge 5804/5804 R/5810/5810 R — Operating manual 2.4 RotorsYou can operate the 5804/5804R/5810/5810R centrifuge with the following rotors.

Centrifuge 5804/5804 R/5810/5810 R — Operating manual14 Blood collection tube5810 748.003flat 3,000 × g3 to 15mL Ø 16mm 4,000rpm16/64 108mm/108

15Centrifuge 5804/5804 R/5810/5810 R — Operating manual Rotor A-4-81 with conical tubesWide-neck bottle, rectangular- flat 3,220×g500mL 83 mm 4,0

Centrifuge 5804/5804 R/5810/5810 R — Operating manual16 Rotor A-4-81 with MTP/Flex carrierMax. g-force: 2,900×gRotor A-4-81 MTP/Flex buckets Max.

17Centrifuge 5804/5804 R/5810/5810 R — Operating manual Slides CombiSlide5825 706.005flat 1,000×g12slides - 2,372rpm12/48 60mm 15.9 cmCell cult

Centrifuge 5804/5804 R/5810/5810 R — Operating manual18 2.4.2 Rotor A-4-62 and A-4-62-MTP (only 5810/5810 R)Rotor A-4-62 with 250 mL rectangular buc

19Centrifuge 5804/5804 R/5810/5810 R — Operating manual Vessel5810 756.006flat 3,050×g7 to 18mL Ø 20mm 4,000rpm8/32 119mm/126mm 17.3 cmVessel

5820 900.040-02/122012 Copyright © 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copy

Centrifuge 5804/5804 R/5810/5810 R — Operating manual20 Rotor A-4-62 with MTP carrierBottles5810 770.009flat 3,220×g180 to 250mL Ø 62mm 4,000rp

21Centrifuge 5804/5804 R/5810/5810 R — Operating manual 96-well PCR plate 5825 711.009flat 2,600×g4,000rpm1/4 53mm 15.2 cmSlides CombiSlide5825

Centrifuge 5804/5804 R/5810/5810 R — Operating manual22 2.4.3 Rotor A-4-44Max. g-force: 4,400×gRotor A-4-44 Rectangular bucket 100mLAerosol-tight

23Centrifuge 5804/5804 R/5810/5810 R — Operating manual Conical tube5804 717.007conical 4,400×g15mL Ø 17.5mm 5,000rpm2/8 121mm/121mm 15.7 cmV

Centrifuge 5804/5804 R/5810/5810 R — Operating manual24 2.4.4 Rotor A-2-DWP-AT (only 5810/5810 R)Vessel5804 760.000flat 4,200×g50 to 75mL Ø 35mm

25Centrifuge 5804/5804 R/5810/5810 R — Operating manual Kit - 3,486×g4,500rpm1/4 60 mm 154 mmIsoRack open 2,500×g24×0.5mL micro test tubesØ

Centrifuge 5804/5804 R/5810/5810 R — Operating manual26 2.4.5 Rotor A-2-DWPHint!Check the load if using two fully loaded DWP plates.Max. g-force: 2,

27Centrifuge 5804/5804 R/5810/5810 R — Operating manual *) Optional. Secures the plate against slipping .384-well PCR plate5825 713.001flat 2,170×

Centrifuge 5804/5804 R/5810/5810 R — Operating manual28 2.4.6 Rotor FA-45-6-30Rotor FA-45-6-30Fixed-angle rotor for 6conical tubesMax. g-force: 16,

29Centrifuge 5804/5804 R/5810/5810 R — Operating manual 2.4.7 Rotor F-34-6-38Vessel5820 728.000Round 16,233×g 5.5mL – 10mL Ø 16mm 19,246 ×g1/6

You will find a detailed description of these figures in your language in Chapters 2.1 and 5.1.Abb. 1: Depiction of Centrifuge 5810 and 5810R. The Ce

Centrifuge 5804/5804 R/5810/5810 R — Operating manual30 Blood collection tube5804 739.000Round 15,442×g4 mL to 7mL Ø 13mm 18,353×g3/18 107mm

31Centrifuge 5804/5804 R/5810/5810 R — Operating manual 2.4.8 Rotors FA-45-30-11 and F-45-30-11Rotor FA-45-30-11Aerosol-tight fixed-angle rotor for

Centrifuge 5804/5804 R/5810/5810 R — Operating manual32 2.4.9 Rotor F-45-48-PCR2.4.10 Rotor T-60-11Rotor F-45-48-PCRFixed-angle rotor for tube strip

33Centrifuge 5804/5804 R/5810/5810 R — Operating manual 2.4.11 Rotor S-4-104 (only 5810/5810 R)Max. g-force: 3,214×gRotor S-4-104 Round bucket 750

Centrifuge 5804/5804 R/5810/5810 R — Operating manual34 Conical tube5825 733.002conical 3,180×g50mL Ø 29mm× 109mm 3,900rpm7/28 116mm/122mm

35Centrifuge 5804/5804 R/5810/5810 R — Operating manual Max. g-force: 2,568×gRotor S-4-104 Plate bucket Aerosol-tight cap Max. speed: 3,900rpmSwi

Centrifuge 5804/5804 R/5810/5810 R — Operating manual36 2.4.12 Rotor S-4-72Slides CombiSlide5825 706.005flat 1,000×g12slides 2.467rpm12/48 47mm

37Centrifuge 5804/5804 R/5810/5810 R — Operating manual Conical tube5804 783.000conical 3,234×g15mL Ø 17mm × 104mm 4,200rpm8/32 120 mm 16.4 cm

Centrifuge 5804/5804 R/5810/5810 R — Operating manual38 2.4.13 Rotor F-35-48-17Max. g-force: 5,005 ×gRotor F-35-48-17 Max. speed: 5,500rpmFixed-an

39Centrifuge 5804/5804 R/5810/5810 R — Operating manual 2.4.14 Rotor FA-45-48-11Max. g-force: 19,083 ×gRotor FA-45-48-11 Max. speed: 13,000rpmAero

Abb. 2: Control panel of the Centrifuge 5804/5810 and theCentrifuge 5804R/5810R.Control panel of the Centrifuge 5804/5810 and theCentrifuge 5804R/5

Centrifuge 5804/5804 R/5810/5810 R — Operating manual40 2.4.15 Rotor FA-45-20-17Max. g-force: 20,913 ×gRotor FA-45-20-17 Max. speed: 13,100rpmAero

41Centrifuge 5804/5804 R/5810/5810 R — Operating manual 3 Safety3 Safety3.1 Intended useThe 5804/5804R/5810/5810R centrifuge is intended exclusive

Centrifuge 5804/5804 R/5810/5810 R — Operating manual42 For all other rotors and rotor lids of this centrifuge there is no service life limit as lon

43Centrifuge 5804/5804 R/5810/5810 R — Operating manual 3.5 Warnings for intended useRead the operating manual and observe the following general saf

Centrifuge 5804/5804 R/5810/5810 R — Operating manual44 3.5.2 Incorrect handling of the centrifugeWARNING!A defective gas spring is an insufficient

45Centrifuge 5804/5804 R/5810/5810 R — Operating manual 3.5.3 Incorrect handling of the rotorsWARNING!Risk of injury from improperly attached rotors

Centrifuge 5804/5804 R/5810/5810 R — Operating manual46 3.5.4 Extreme strain on the centrifuging tubesCAUTION!Risk of injury from overloaded tubes.

47Centrifuge 5804/5804 R/5810/5810 R — Operating manual 3.5.5 Aerosol-tight centrifugation3.6 Safety instructions located on the deviceWARNING!Risk

Centrifuge 5804/5804 R/5810/5810 R — Operating manual48 4 Installation4 Installation4.1 Selecting the locationSelect the location for the device acc

49Centrifuge 5804/5804 R/5810/5810 R — Operating manual 4.3 Installing the instrumentPerform the following steps in the sequence described.1. Allow

5Centrifuge 5804/5804 R/5810/5810 R — Operating manual Operating manual — Centrifuge 5804/5804 R/5810/5810 Rseeon p.Fig.Tab.p.Operating manual1 T

Centrifuge 5804/5804 R/5810/5810 R — Operating manual50 5 Operation5 Operation5.1 Overview of operating controlsBefore using the centrifuge for the

51Centrifuge 5804/5804 R/5810/5810 R — Operating manual 5.2 Preparing for centrifugation5.2.1 Switching on the centrifuge1. Switch on the centrifuge

Centrifuge 5804/5804 R/5810/5810 R — Operating manual52 5.2.4 Loading the rotorFixed-angle rotorsTo load the rotor, proceed as follows:1. Check the

53Centrifuge 5804/5804 R/5810/5810 R — Operating manual Swing-bucket rotorPrerequisites• A combination of rotor, carrier and adapter, approved by Ep

Centrifuge 5804/5804 R/5810/5810 R — Operating manual54 Abb. 5: Loading the adapter 16mm75 - 100mm with tubes >119mm in length.Fig. 5: Loadi

55Centrifuge 5804/5804 R/5810/5810 R — Operating manual 5.2.5 Closing the centrifuge lid1. Check the correct attachment of the rotor and rotor lid.2

Centrifuge 5804/5804 R/5810/5810 R — Operating manual56 temperature, 4°C must be met via this continuous cooling in order to prevent the rotor cha

57Centrifuge 5804/5804 R/5810/5810 R — Operating manual 5.4 CentrifugingEach of the centrifuging variants described here must be preceded by the pre

Centrifuge 5804/5804 R/5810/5810 R — Operating manual58 5.4.2 Centrifuging in continuous operationPerform the following steps in the sequence descri

59Centrifuge 5804/5804 R/5810/5810 R — Operating manual 5.4.4 Removing the rotorPrerequisitesWhen attaching the rotor to or releasing it from the mo

Centrifuge 5804/5804 R/5810/5810 R — Operating manual6 5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Centrifuge 5804/5804 R/5810/5810 R — Operating manual60 6 Operating controls and function6 Operating controls and function6.1 Setting the radiusWhen

61Centrifuge 5804/5804 R/5810/5810 R — Operating manual 6.4 Saving the programYou can save the current centrifugation parameters and functions (At s

Centrifuge 5804/5804 R/5810/5810 R — Operating manual62 6.7 Special functions6.7.1 Display operating hoursRequirementRotor stop.6.7.2 Switching on/o

63Centrifuge 5804/5804 R/5810/5810 R — Operating manual 7 Maintenance7 Maintenance7.1 MaintenanceWe recommend that the centrifuge with the associate

Centrifuge 5804/5804 R/5810/5810 R — Operating manual64 7.3 Cleaning/disinfectionDANGER!Electric shock as a result of penetration of liquid. Switch

65Centrifuge 5804/5804 R/5810/5810 R — Operating manual 7.3.1 Cleaning and disinfecting the device1. Open the lid. Switch off the device with the ma

Centrifuge 5804/5804 R/5810/5810 R — Operating manual66 7.4 Additional service instructions for Centrifuge 5804R/5810R Empty and clean the conden

67Centrifuge 5804/5804 R/5810/5810 R — Operating manual 8 Troubleshooting8 TroubleshootingIf you cannot remedy an error with the recommended measure

Centrifuge 5804/5804 R/5810/5810 R — Operating manual68 No rotorCentrifuge does not start up.Error in the drive or in the rotor detection. Switch t

69Centrifuge 5804/5804 R/5810/5810 R — Operating manual 8.4 Emergency releaseIf the centrifuge lid cannot be opened, you can activate the emergency

7Centrifuge 5804/5804 R/5810/5810 R — Operating manual 10 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Centrifuge 5804/5804 R/5810/5810 R — Operating manual70 9 Transport, storage and disposal9 Transport, storage and disposal9.1 Transport Remove the

71Centrifuge 5804/5804 R/5810/5810 R — Operating manual 10 Technical data10 Technical data10.1 Power supply10.2 Ambient conditions10.3 Weight/dimens

Centrifuge 5804/5804 R/5810/5810 R — Operating manual72 The noise level was measured according to DIN EN ISO 3745 frontally in a sound measuring roo

73Centrifuge 5804/5804 R/5810/5810 R — Operating manual Deceleration times according to DIN 58 970Tab. 2: Approximate deceleration times of the dif

Centrifuge 5804/5804 R/5810/5810 R — Operating manual74 11 Ordering information11 Ordering information11.1 Centrifuge 5804/5804 RPlease refer to our

75Centrifuge 5804/5804 R/5810/5810 R — Operating manual Rotor A-4-81, MTP/Flex carrier5810 745.0045810 746.0005810 720.0015825 717.0075810 748.00358

Centrifuge 5804/5804 R/5810/5810 R — Operating manual76 Rotor A-4-81, bucket for conical tubes11.3.2 Rotor A-4-62 and A-4-62-MTP (only 5810/5810 R)R

77Centrifuge 5804/5804 R/5810/5810 R — Operating manual Rotor A-4-62-MTP11.3.3 Rotor A-4-445810 781.000 022662431Replacement clampfor adapters for 2

Centrifuge 5804/5804 R/5810/5810 R — Operating manual78 11.3.4 Rotor A-2-DWP-AT (only 5810/5810 R)Aerosol impermeability tested and certified by the

79Centrifuge 5804/5804 R/5810/5810 R — Operating manual 11.3.6 Rotor FA-45-6-30Aerosol impermeability tested and certified by the Centre of Emergenc

Centrifuge 5804/5804 R/5810/5810 R — Operating manual8 ENOperating manual

Centrifuge 5804/5804 R/5810/5810 R — Operating manual80 11.3.8 Rotor FA-45-30-11 and rotor F-45-30-1111.3.9 Rotor F-45-48-PCR11.3.10 Rotor T-60-1158

81Centrifuge 5804/5804 R/5810/5810 R — Operating manual 11.3.11 Rotor S-4-104Order no. (international)Order No. (North America)Description5820 740.0

Centrifuge 5804/5804 R/5810/5810 R — Operating manual82 11.3.12 Rotor S-4-7211.3.13 Rotor F-35-48-1711.3.14 FA-45-48-11 rotor5820 751.002 5820751002

83Centrifuge 5804/5804 R/5810/5810 R — Operating manual 11.3.15 Rotor FA-45-20-1711.3.16 Miscellaneous5820 767.006 5820767006Seal for rotor lidFA-45

Centrifuge 5804/5804 R/5810/5810 R — Operating manual84 Power cable for Centrifuge 5804 and Centrifuge 5810Power cable for Centrifuge 5804 R and Cen

9Centrifuge 5804/5804 R/5810/5810 R — Operating manual 1 User instructions1 User instructions1.1 Using this manual Read this operating manual compl

Your local distributor: www.eppendorf.com/worldwideEppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 53801-0 · Fax: +49 40 538 01-556 · E-mail: epp