Eppendorf Reference User Manual download pdf (Page 20)

Error Cause Solution

Droplets on

the inner wall

of the pipette

tip.

– Uneven wetting of the

plastic wall.

– A pipette tip with poor

wetting properties has

been used.

– Attach a new pipette tip.

– Use an original eppendorf

tip.

Pipette is

dripping

and/or the

volume

pipetted is

incorrect.

– The tip is loose.

– A poorly fitting pipette tip

has been used.

– Liquid with a high vapor

pressure has been pipetted.

– Tip has been taken out of

the liquid too quickly.

The pipette is dripping

because:

– Piston is contaminated.

– Piston is damaged.

– Seals are damaged.

– Tip cone loose.

– Press the tip firmly in place.

– Use an original eppendorf

tip.

– In this case, we recommend

pipetting using a positive-

displacement pipette.

– Remove the tip slowly from

the liquid.

– Clean and lightly lubricate

the piston.

– Replace the piston and seal

(see Part B, Maintenance).

– Replace all seals

(see Part B, Maintenance).

– Lightly tighten tip cone with

wrench (see Part B,

Maintenance).

Exchange, where

necessary.

10 Troubleshooting

Eppendorf Reference® – Part A – Chapter 10

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Eppendorf Reference User Manual Page 20

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