Eppendorf SOP User Manual download pdf (Page 8)

Calibration

Eppendorf SOP

English (EN)

2 Calibration

2.1 Scope of testing

Note concerning the nominal volume:

The nominal volume of an adjustable-volume pipette is the largest volume to be

set by the user and specified by the manufacturer.

When using the Combitip with the Multipette, the nominal volume is the largest

possible dispensing volume of the Combitip. This means that for the Multipette

plus and Multipette M4, the nominal volume is 1/5 of the filling volume of the

Combitip advanced. For the Multipette stream / Xstream, the nominal volume is

the filling volume of the Combitip advanced.

2.1.1 Adjusting adjustable volume pipettes

For variable pipettes, check 3 different volumes with 10 measured values each:

• the nominal volume,

• approx. 50 % of the nominal volume,

• 10 % of the nominal volume.

2.1.2 Multi-channel pipettes

For Multi-channel pipettes check each channel separately with 3 different

volumes, each with 10 measured values:

• the nominal volume,

• approx. 50 % of the nominal volume,

• 10 % of the nominal volume.

2.1.3 Multipette

With the

• Multipette,

• Multipetteplus,

• Multipette stream,

• MultipetteXstream

• MultipetteM4

check the nominal volume with 10 measured values with the Eppendorf

Combitip used.

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Eppendorf SOP User Manual Page 8