Eppendorf SOP User Manual Page 1

rating Procedure for PipettesOPEN)Operating Procedure for Pipettes Register your instrument! www.eppendorf.com/myeppendorfEppendorf SOPStandard Operat

10CalibrationEppendorf SOPEnglish (EN)2.2.3 Dispensing the test volume into the weighing vessel1. Rest the filled tip up against the wall of the weigh

11EvaluationEppendorf SOPEnglish (EN)3 Evaluation3.1 Calculating the systematic errorMean value of the dispensed volume:n = Number of measured valuesT

12Sterilization and cleaningEppendorf SOPEnglish (EN)4 Sterilization and cleaning4.1 SterilizationParameter for autoclaving• 121 °C• 20 minutes• 1 bar

13Sterilization and cleaningEppendorf SOPEnglish (EN)4.1.3 Research and Research proWith the Research and Research pro pipettes, the lower section is

14Sterilization and cleaningEppendorf SOPEnglish (EN)4.1.7 Xplorer and Xplorer plusThe lower part of theXplorer and Xplorer plus pipettes can be autoc

15Leakage checkEppendorf SOPEnglish (EN)5 Leakage check5.1 Pipette leakage checkIn order to carry out a leakage check on a pipette, follow the procedu

16TroubleshootingEppendorf SOPEnglish (EN)Liquid cannot be aspirated correctly or dripping occurs. • Leakage in the Combitip advanced.  Replace the C

17AdjustmentEppendorf SOPEnglish (EN)7Adjustment7.1 General informationPrior to delivery, all pipettes are adjusted with distilled or de-ionized, dega

18AdjustmentEppendorf SOPEnglish (EN)7.2.1 Procedure Requirements Device, original Eppendorf tip, test liquid and ambient air must have the same tempe

19AdjustmentEppendorf SOPEnglish (EN)7.2.2 BiomasterAuxiliary equipment • Supplied pipette wrench (order no. 4910 092.001) 7.2.3 Reference variableAux

AESOP13640-08/052013Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyrigh

20AdjustmentEppendorf SOPEnglish (EN)7.2.4 Reference 2 adjustable-volume7.2.4.1 User adjustmentChanging the adjustment will change the volume by a spe

21AdjustmentEppendorf SOPEnglish (EN)7. Attach the red adjustment seal after the inspections.If the adjustment only applies to a specific liquid, mark

22AdjustmentEppendorf SOPEnglish (EN)1. Check whether the adjustment display is set to "0".If the adjustment display is not set to “0”, it m

23AdjustmentEppendorf SOPEnglish (EN)5. Turn the adjustment tool to set the volume display. Set the volume display (from the volume setting used durin

24AdjustmentEppendorf SOPEnglish (EN)7.2.5 Research variable Auxiliary equipment • Supplied pipette wrench (order no. 3111 501.016) • Supplied red CAL

25AdjustmentEppendorf SOPEnglish (EN)7.2.6 Research plusadjustable volume - adjustment to environmental parametersAdjustment change for specific liqui

26AdjustmentEppendorf SOPEnglish (EN)7.2.7 Research plus adjustable volume - change to the factory adjustmentAuxiliary equipment • Supplied safety plu

27AdjustmentEppendorf SOPEnglish (EN)2. Provide the volume setting ring and the ejector with a common mark with a pen. This mark is used for orientati

28AdjustmentEppendorf SOPEnglish (EN)4. Continue to keep the ejector pressed. Insert the safety plug tool in such a way that the counter locking mecha

29AdjustmentEppendorf SOPEnglish (EN)6. This results in the following approximate volume changes:The values mentioned are theoretical values and are f

3Table of contentsEppendorf SOPEnglish (EN)Table of contents1 Test and pipette conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

30AdjustmentEppendorf SOPEnglish (EN)9. Document the changes made and the measurements conducted. Remove the mark on the volume setting ring and on th

31AdjustmentEppendorf SOPEnglish (EN)7.2.8 Research Multi-channel Auxiliary equipment • Supplied pipette wrench (order no. 3111 501.016) •Supplied red

32AdjustmentEppendorf SOPEnglish (EN)7.2.10 Varispenser plus 7.2.11 Xplorer and Xplorer plus Turn the fine adjustment toward + or –. A revolution cor

33AdjustmentEppendorf SOPEnglish (EN)7.3 Adjusting fixed-volume pipettes The adjustment process for fixed-volume pipettes is identical to the adjustm

34AdjustmentEppendorf SOPEnglish (EN)7.3.2 Reference2Adjustment is carried out as described for the Reference 2 adjustable-volume (see p. 20). 7.3.3 R

35AdjustmentEppendorf SOPEnglish (EN)7.3.4 Research plus fixed Adjustment is carried out as described for the Research plus adjustable-volume(see p. 2

36Factor Z for distilled waterEppendorf SOPEnglish (EN)8 Factor Z for distilled water8.1 Factor Z overview tableFactor Z (µl/mg) in accordance with EN

37Technical specificationsEppendorf SOPEnglish (EN)9 Technical specificationsThe following specifications are valid for the following conditions:9.1 F

38Technical specificationsEppendorf SOPEnglish (EN)9.1.2 Reference 2 fixedFixed-volume Reference2 Model Test tipepT.I.P.S. Color codeVolume rangeLengt

39Technical specificationsEppendorf SOPEnglish (EN)9.1.3 Research fixedModel Test tipepT.I.P.S. Color codeVolume rangeLengthError limitsErrorSystemati

4Table of contentsEppendorf SOPEnglish (EN)5 Leakage check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

40Technical specificationsEppendorf SOPEnglish (EN)9.1.4 Research plus fixedResearch plus fixed volumeModel Test tipepT.I.P.S. Color codeVolume rangeL

41Technical specificationsEppendorf SOPEnglish (EN)9.2 Adjusting adjustable volume pipettes 9.2.1 Reference variable Model Test tipepT.I.P.S. Color c

42Technical specificationsEppendorf SOPEnglish (EN)9.2.2 Reference 2 adjustable-volume Reference2 single-channel variableModel Test tipepT.I.P.S. Colo

43Technical specificationsEppendorf SOPEnglish (EN)Reference2 single-channel variableModel Test tipepT.I.P.S. Color codeVolume rangeLengthTesting volu

44Technical specificationsEppendorf SOPEnglish (EN)9.2.3 Research adjustable-volume Model Test tipepT.I.P.S. Color codeVolume rangeLengthTesting volum

45Technical specificationsEppendorf SOPEnglish (EN)9.2.4 Research proModel Test tipepT.I.P.S. Color codeVolume rangeLengthTesting volumeError limitsEr

46Technical specificationsEppendorf SOPEnglish (EN)9.2.5 Research plus adjustable-volume Research plus adjustable single-channelModel Test tipepT.I.P.

47Technical specificationsEppendorf SOPEnglish (EN)9.2.6 Biomaster9.2.7 Varipette0.5 µL – 5 mLIncrement:0.005 mLPurple0.1 µL – 5 mL120 mm0.5 mL ±2.4

48Technical specificationsEppendorf SOPEnglish (EN)9.2.8 Xplorer and Xplorer pluspipetteIncrementPipette tipColor codeVolume rangeLengthTesting volume

49Technical specificationsEppendorf SOPEnglish (EN)9.3 Multi-channel pipettes 9.3.1 ResearchModel Test tipepT.I.P.S. Color codeVolume rangeLengthVol

5Table of contentsEppendorf SOPEnglish (EN)9 Technical specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

50Technical specificationsEppendorf SOPEnglish (EN)9.3.2 Research proModel Test tipepT.I.P.S. Color codeVolume rangeLengthTesting volumeError limitsEr

51Technical specificationsEppendorf SOPEnglish (EN)9.3.3 Research plusResearch plus adjustable multi-channelModel Test tipepT.I.P.S. Color codeVolume

52Technical specificationsEppendorf SOPEnglish (EN)9.3.4 Reference2Reference2 Variable multi-channel (8-channel/12-channel)Model Test tipepT.I.P.S. Co

53Technical specificationsEppendorf SOPEnglish (EN)9.3.5 Xplorer and Xplorer pluspipetteIncrementPipette tipColor codeVolume rangeLengthTesting volume

54Technical specificationsEppendorf SOPEnglish (EN)9.4 Multipette The following specifications for the Multipette plus, Multipette stream / Multipett

55Technical specificationsEppendorf SOPEnglish (EN)9.4.2 MultipetteM4Combitip advanced Testing volume Error limitsErrorSystematic error Random error±

56Technical specificationsEppendorf SOPEnglish (EN)9.4.3 Multipette stream / Multipette XstreamCombitip advancedVolume range Testing volumeError limit

57Technical specificationsEppendorf SOPEnglish (EN)9.5 Varispenser / Top Buret The following specifications for Varispenser and Top Buret are vali

58Technical specificationsEppendorf SOPEnglish (EN)9.6.1 Air-cushion pipettes, fixed and adjustable volume9.6.2 Positive displacement pipettes (Biomas

59Technical specificationsEppendorf SOPEnglish (EN)9.6.3 Dispenser (Multipette)Nominal volume Error limitsMeasurement errorsSystematic Random± % ± µL

6Test and pipette conditionsEppendorf SOPEnglish (EN)1 Test and pipette conditionsFor the calibration or adjustment of pipettes, the scales and measur

60Technical specificationsEppendorf SOPEnglish (EN)9.6.4 Single-stroke dispenser (Varispenser)9.6.5 Piston stroke buretsNominal volume Error limitsMea

61IndexEppendorf SOPEnglish (EN)IndexAAccuracy... 6Adjustable-volume pipettesAdjust...

62IndexEppendorf SOPEnglish (EN)RRandom error... 11SScope of testingAdjusting adjustable volume pipettes ...

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7Test and pipette conditionsEppendorf SOPEnglish (EN)1.2 Measuring station1.2.1 EvaporationTake evaporation protection into account while carrying out

8CalibrationEppendorf SOPEnglish (EN)2 Calibration2.1 Scope of testingNote concerning the nominal volume:The nominal volume of an adjustable-volume pi

9CalibrationEppendorf SOPEnglish (EN)2.1.4 Bottle-top dispenser and Top Buret For bottle-top dispensers and the Top Buret, check the nominal volume wi