Eppendorf SOP User Manual download pdf (Page 10)

Calibration

Eppendorf SOP

English (EN)

2.2.3 Dispensing the test volume into the weighing vessel

1. Rest the filled tip up against the wall of the weighing vessel at an angle.

2. Dispense the test liquid slowly until the first stop (measuring stroke).

3. Press the control button to the second stop (blow-out) and dispense the

remaining liquid in the tip (does not apply for dispensers and burets).

4. Hold down the control button and pull the tip up the vessel wall.

5. Let the control button slide back into position.

6. Determine the weight.

7. Complete all measurements of a measuring series as described and calculate

the systematic and random error (see p. 11).

8. In case of adjustable-volume pipettes, determine the measurement with the

nominal volume, 50 % and 10 % of the nominal volume. Always begin the

test with 10 % of the nominal volume.

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Eppendorf SOP User Manual Page 10