Eppendorf SOP User Manual download pdf (Page 12)

Sterilization and cleaning

Eppendorf SOP

English (EN)

4 Sterilization and cleaning

4.1 Sterilization

Parameter for autoclaving

• 121 °C

• 20 minutes

• 1 bar overpressure

4.1.1 Reference and Biomaster

The Reference pipettes and the Biomaster are fully autoclavable.

1. Prior to autoclaving, unscrew the upper and lower part of the pipette apart by

approx. one turn to enable the vapor to enter more easily.

2. After autoclaving, allow the pipette to cool down to room temperature and to

dry completely and then screw together.

4.1.2 Research plus and Reference 2

The Research plus pipettes and Reference 2 pipettes are fully autoclavable.

1. You can put the Research plus and Reference 2 into the autoclave as a

complete unit or with the lower part removed. Do not disassemble the lower

part.

2. For 2.5 mL, 5 mL and 10 mL pipettes: remove the old protection filter. Add a

new protection filter and install it after autoclaving. Only autoclave the

protection filter once.

After autoclaving:

3. Cool the pipette down to room temperature and leave to dry.

4. For 2.5 mL, 5 mL / 10 mL pipettes: the protection filter swells during

autoclaving. Slightly squeeze the protection filter when installing it into the

cone tip.

Further notes on cleaning and sterilization can found in the relevant

operating manual.

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Eppendorf SOP User Manual Page 12