Eppendorf SOP User Manual download pdf (Page 6)

Test and pipette conditions

Eppendorf SOP

English (EN)

1 Test and pipette conditions

For the calibration or adjustment of pipettes, the scales and measuring station

should fulfill the following requirements:

1.1 Balances

1.1.1 Balance type

Use semi-microbalances and microbalances to calibrate pipettes.

Some companies offer balances that are specially designed to meet the

requirements of pipette calibration, e.g. Sartorius and Mettler.

1.1.2 Accuracy

When selecting the balances, ensure that they are suitable for the accuracy of

the pipette. This helps to ensure that deviations within a measuring series are

recorded exactly for the assessment of the systematic and random measurement

deviations in accordance with EN ISO 8655.

For a pipette volume of less than or equal to 10 µl, balances with a 6-digit

display must be used. With larger volumes, balances with a 5-digit display are

sufficient.

1.1.3 Minimum requirements for balances

* For practical reasons, the nominal volume can be used for the selection of the

balances.

These test and pipetting conditions are valid for all Eppendorf pipettes

and dispensers.

Follow EN ISO 8655 "Volume-measuring equipment with pistons".

Selected volume* of the pipette to be

tested

Display resolution (mg)

1 µL to 10 µL 0.001

>10 µL to 100 µL 0.01

>100 µL to 1 000 µL 0.1

>1 mL to 10 mL 0.1

>10 mL to 200 mL 1

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Eppendorf SOP User Manual Page 6