Eppendorf 5430 R Centrifuge User Manual download pdf (Page 36)

Operation

Centrifuge 5430/5430 R

English (EN)

5.5 Cooling (only 5430 R)

5.5.1 Temperature adjustment

 Set the temperature using the temp arrow keys between -11°C and +40°C.

You can also modify the temperature during centrifugation.

At higher ambient temperature a brief fan noise is possible until the desired temperature has been

reached. This indicates a heavy cooling performance.

5.5.2 Temperature display

The set temperature appears in the extended display.

5.5.3 Temperature monitoring

After the target temperature has been reached, the centrifuge reacts to temperature deviations during

centrifugation as follows:

5.5.4 FastTemp

This function can be used to start a temperature control run directly without samples with a rotor and

temperature-specific speed in order to quickly adjust the rotor chamber, including the rotor, buckets and

adapters, to the previously set nominal temperature.

The FastTemp pro function for programming the temperature control run with defined start times is

described in the next section.

Prerequisites

• The centrifuge is switched on.

• The rotor and rotor lid are properly attached.

• The centrifuge lid is closed.

• Temperature and g-force (rcf)/speed (rpm) for the centrifugation are set (see Centrifuging on p. 39).

1. Press the fast temp key.

The display shows Fast Temp, the remaining duration of the temperature control run as well as the

current temperature and g-force (rcf)/speed (rpm).

The temperature control run automatically ends when the set temperature is reached. A periodic signal

tone sounds.

2. Press the start/stop key to terminate the temperature control run early.

After the set temperature has been reached and the temperature control run is complete, the centrifuge

keeps the rotor chamber with the centrifuge lid closed at the set target temperature if the temperature is

below the ambient temperature. However, independent of the target temperature, 4 °C must be met via

this continuous cooling in order to prevent the rotor chamber from freezing.

If the rotor is stopped: Target temperature

During centrifugation: Actual temperature

Deviation from set value Action

± 3 °C Temperatures on the display are flashing.

± 5 °C Periodic warning tone and display Error 18. Centrifugation is stopped

automatically.

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Eppendorf 5430 R Centrifuge User Manual Page 36