Eppendorf Pipette Reference 2 User Manual download pdf (Page 28)

Maintenance

Eppendorf Reference

English (EN)

6.5 Adjusting pipettes

6.5.1 General instructions on user and factory adjustment

The Reference 2 was adjusted and tested prior to delivery. The adjustment opening is sealed using a gray

plastic adjustment seal. The adjustment seal is labeled with the abbreviation "ADJ". The adjustment display

shows "0".

Changing the adjustment of the Reference 2 is sometimes recommended for solutions that are very

different from water in terms of their density, viscosity, surface tension and/or vapor pressure, etc. If the

density of an aqueous solution changes by approximately ±10

%, for example, due to the salt

concentration, the volume changes by approximately ±0.2

%. This statement does not apply if other

relevant properties of the liquid also change.

If the pipette is used at extremely high altitudes, it must be calibrated to the ambient air pressure. At

1000 m above sea level, the volume error of a 100 µL pipette is about -0.3

When using special tips, i.e. tips with a geometry that is very different from standard tips, changing the

adjustment can improve the dispensing accuracy (systematic error).

Follow the adjustment tips for epT.I.P.S. long provided in the User adjustment document on the Reference 2

CD.

Adjustment changes can be reset using simple steps.

In addition to changing the user adjustment, a Reference 2 can be permanently changed by altering the

factory adjustment.

Changes to the user or factory adjustment do not affect the dispensing precision (random error). Precision

can be improved by exchanging worn parts. Precision is also significantly affected by handling.

NOTICE! Incorrect dispensing volume due to differences in temperature and for special

liquids.

Temperature differences between the pipette, pipette tips and liquid; or solutions with

physical data that is very different from that of water; may result in incorrect dispensing

volumes.

 Avoid temperature differences the between pipette, pipette tip and liquid.

 Ensure that the temperature is between 20 °C and 27 °C and remains constant at ±0.5 °C.

 Check the dispensing volume and make sure that you can affirm all the questions listed in

the general information.

The random and systematic errors recorded on delivery can be found in the Eppendorf

Certificate. This certificate is included in delivery. Changes to the factory adjustment will

render the certificate void.

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Eppendorf Pipette Reference 2 User Manual Page 28