Eppendorf Pipette Reference 2 User Manual Page 1

nualeference® 2EN)g manual Register your instrument! www.eppendorf.com/myeppendorfEppendorf Reference® 2Operating manual

Product descriptionEppendorf Reference® 2English (EN)102.3.2 Reference 2 Multi-channelFor an explanation of the upper part of the pipette, refer to th

Product descriptionEppendorf Reference® 2English (EN)112.4 MaterialsThe Reference 2 components that can be accessed by the user are made of the follow

Product descriptionEppendorf Reference® 2English (EN)122.5 Storing the pipetteThe pipette can be safely stored in a pipette carousel or a wall mountin

SafetyEppendorf Reference® 2English (EN)133 Safety3.1 Intended use3.2 Warnings for intended useThe intended use is described in a separate document en

SafetyEppendorf Reference® 2English (EN)14NOTICE! Damage to device from missing pipette tips. Use the Reference 2 with fitted pipette tips only.  Wh

OperationEppendorf Reference® 2English (EN)154 Operation4.1 Setting the volume (only Reference 2 variable)4.2 Using pipette tipsThe liquid to be dispe

OperationEppendorf Reference® 2English (EN)164.3 Deactivating the tip cone spring loadingFor the following dispensing tasks, it may be helpful to deac

TroubleshootingEppendorf Reference® 2English (EN)175 Troubleshooting5.1 Error searchSymptom/message Cause RemedyLiquid is dripping from the tip and/or

TroubleshootingEppendorf Reference® 2English (EN)18Regularly check the precision and accuracy of the Reference 2 to prevent dispensing errors. You can

MaintenanceEppendorf Reference® 2English (EN)196 Maintenance• All single and multi-channel lower parts are wear parts. Clean them after contamination,

4920 900.017-01/052013 Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyri

MaintenanceEppendorf Reference® 2English (EN)206.2 Sterilizing or disinfecting the pipette6.2.1 AutoclavingAll Reference 2 pipettes are completely ste

MaintenanceEppendorf Reference® 2English (EN)216.2.2 Disinfection Carefully wipe off the outer surfaces with disinfectant, DNA/RNA decontamination ag

MaintenanceEppendorf Reference® 2English (EN)226.4 Disassembling and assembling the pipette lower part6.4.1 Single-channel lower part up to 1000 µL6.4

MaintenanceEppendorf Reference® 2English (EN)236.4.1.3 Opening and assembling the lower part (≤ 1000 µL) To avoid confusion of parts, only disassemble

MaintenanceEppendorf Reference® 2English (EN)246.4.2 Single-channel lower part Applies to the following single-channel pipettes:•2 mL• 2.5 mL•5 mL•10

MaintenanceEppendorf Reference® 2English (EN)256.4.2.2 Opening and assembling the lower part (2 mL or larger) 1. : Unscrew the ejector sleeve.2. : K

MaintenanceEppendorf Reference® 2English (EN)26The lower part consists of the following:Before assembling the pipette, first install the piston mount,

MaintenanceEppendorf Reference® 2English (EN)276.4.3.2 Removing and installing channels1. and : Position a pipette tip under the piston and careful

MaintenanceEppendorf Reference® 2English (EN)286.5 Adjusting pipettes6.5.1 General instructions on user and factory adjustmentThe Reference 2 was adju

MaintenanceEppendorf Reference® 2English (EN)29The existing dispensing volume must be checked before changing the factory calibration or adjustment. T

Table of contentsEppendorf Reference® 2English (EN)3Table of contents1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . .

MaintenanceEppendorf Reference® 2English (EN)306.5.2 Changing the user adjustmentIf the adjustment is changed, the volume changes by a certain value.

MaintenanceEppendorf Reference® 2English (EN)316.5.3 Changing the factory adjustmentAuxiliary aids from the delivery package• Red plastic safety plug•

MaintenanceEppendorf Reference® 2English (EN)326.6 Decontamination before shipmentIf you wish to return the device to Eppendorf AG or an Eppendorf AG

Technical dataEppendorf Reference® 2English (EN)337 Technical data7.1 Reference 2 Single-channel variableReference 2 single-channel variableModel Test

Technical dataEppendorf Reference® 2English (EN)347.2 Reference 2 Multi-channel variableReference 2 single-channel variableModel Test tipepT.I.P.S. co

Technical dataEppendorf Reference® 2English (EN)357.3 Reference 2 fixedReference 2 fixed volumeModel Test tip epT.I.P.S. color codeVolume rangeLengthE

Technical dataEppendorf Reference® 2English (EN)367.4 Test conditions Test conditions and test evaluation match in compliance with ISO 8655, Part 6.

Ordering InformationEppendorf Reference® 2English (EN)378 Ordering Information8.1 Single-channel pipettes, variable volume8.2 Single-channel pipettes,

Ordering InformationEppendorf Reference® 2English (EN)388.3 Single-channel lower part for variable volume and fixed volume8.4 Multi-channel pipettesOr

Ordering InformationEppendorf Reference® 2English (EN)398.5 Multi-channel lower parts8.5.1 8-channel lower parts8.5.2 12-channel lower parts8.6 Spare

Table of contentsEppendorf Reference® 2English (EN)46.5 Adjusting pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Ordering InformationEppendorf Reference® 2English (EN)40

PatentsEppendorf Reference® 2English (EN)419 PatentsU.S. Patent No. 7,434,484 Is used by Reference 2 pipettes with variable volume setting (single-cha

IndexEppendorf Reference® 2English (EN)42IndexAAssembly10 µL, 100 µL and 300 µL multi-channel lower part...

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Operating instructionsEppendorf Reference® 2English (EN)51 Operating instructions1.1 Using this manual Read this operating manual completely before u

Operating instructionsEppendorf Reference® 2English (EN)6

Product descriptionEppendorf Reference® 2English (EN)72 Product description2.1 FeaturesReference 2 pipettes are piston-stroke pipettes which operate a

Product descriptionEppendorf Reference® 2English (EN)82.2 Delivery packageThe following accessories are included in the delivery package:Piece Descrip

Product descriptionEppendorf Reference® 2English (EN)92.3 Main illustration2.3.1 Reference 2 single-channel1 Volume displayVolume display with four