Eppendorf epMotion 5070 User Manual

nual070EN)e manual Register your instrument! www.eppendorf.com/myeppendorfepMotion® 5070Hardware manual

Operating instructionsepMotion® 5070English (EN)10FFilling volumeMaximum liquid volume of a labware. The epMotion uses the gripper to transport the la

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Operating instructionsepMotion® 5070English (EN)11Random errorPrecision. Standard deviation of the average value of the dispensed volumes.Remaining vo

Operating instructionsepMotion® 5070English (EN)12VVesselTube or single well of a plate.WWork volumeLiquid volume of a labware. The epMotion fills a v

Product descriptionepMotion® 5070English (EN)132 Product description2.1 Main illustration2.1.1 epMotion 5070Abb. 2-1: Main illus tration e pMotion 50

Product descriptionepMotion® 5070English (EN)142.1.2 epMotion 5070fAbb. 2-2: Main illus tration e pMotion 5070fFig. 2-2: Main illustration epMotion 5

Product descriptionepMotion® 5070English (EN)152.1.3 InterfacesAbb. 2-3: Interfaces epMotion 50 70 and 5070fFig. 2-3: Interfaces epMotion 5070 and 5

Product descriptionepMotion® 5070English (EN)162.1.4 EasyConAbb. 2-4: Eas yConFig. 2-4: EasyConThe EasyCon is the control panel of the epMotion. The E

Product descriptionepMotion® 5070English (EN)172.2 Delivery packageQuantityOrder no. (International)DescriptionAutomated pipetting system epMotion 507

Product descriptionepMotion® 5070English (EN)182.3 FeaturesThe epMotion 5070 is available with different features. All available features are describe

SafetyepMotion® 5070English (EN)193Safety3.1 Intended use3.1.1 Intended use of the epMotion 5070The device is intended for use in laboratories for res

5070 900.850 -00/062013Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copy

SafetyepMotion® 5070English (EN)203.3 Information on product liabilityIn the following cases, the designated protection of the device may be compromis

SafetyepMotion® 5070English (EN)21WARNING! Damage to health due to infectious liquids and pathogenic germs. When handling infectious liquids and path

SafetyepMotion® 5070English (EN)223.5 Hazard symbols and safety devices on the deviceThis section explains the warning symbols on the epMotion and the

InstallationepMotion® 5070English (EN)234 Installation4.1 Selecting the locationInformation on ambient conditions, dimensions and weights can be found

InstallationepMotion® 5070English (EN)24

HardwareepMotion® 5070English (EN)255Hardware5.1 Worktable for epMotion 5070 and 5070fAbb. 5-1: Worktable for epMotionFig. 5-1: Worktable for epMotion

HardwareepMotion® 5070English (EN)265.2 Optical sensorAbb. 5-2: Optical sensor principleFig. 5-2: Optical sensor principleOn the carrier, the optical

HardwareepMotion® 5070English (EN)275.3 ToolsThe tool holder picks up a dispensing tool and puts it down again. The tool holder recognizes the tool fr

HardwareepMotion® 5070English (EN)28

Dispensing liquidsepMotion® 5070English (EN)296 Dispensing liquids6.1 Basic procedure for dispensing6.1.1 Operating principle of dispensing toolsAbb.

Table of contentsepMotion® 5070English (EN)3Table of contents1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Dispensing liquidsepMotion® 5070English (EN)306.1.2 Liquid aspiration up to the remaining volume Abb. 6-2: Liquid aspiration up to the rem aining volu

Dispensing liquidsepMotion® 5070English (EN)316.1.4 Transporting and dispensing liquidAbb. 6-3: Transporting and dispensing li quidFig. 6-3: Transport

Dispensing liquidsepMotion® 5070English (EN)326.2 Dispensing modesThe epMotion can be used to dispense liquids in the Pipette and Multidispense modes.

Dispensing liquidsepMotion® 5070English (EN)33The volume for the additional aspriation depends on the dispensing tool; the software will take it into

Dispensing liquidsepMotion® 5070English (EN)346.2.3.1 Recommended mixing speedsYou can set mixing speeds of 0.2 mm/s – 110 mm/s. 6.3 Special features6

Dispensing liquidsepMotion® 5070English (EN)356.3.2 Liquid aspiration from high vesselsObserve the following special features when using high vessels,

Dispensing liquidsepMotion® 5070English (EN)366.3.3 Liquid dispensing at the height of the vessel edge In the software, the pipette tip can be positio

Labware – vessels, plates and pipette tipsepMotion® 5070English (EN)377 Labware – vessels, plates and pipette tipsThe term labware refers to epMotion

Labware – vessels, plates and pipette tipsepMotion® 5070English (EN)387.2 PlatesAbb. 7-2: Plates: left 96-well MTP, middle 2 4-well MTP, right 96-well

Labware – vessels, plates and pipette tipsepMotion® 5070English (EN)397.2.2 MicroplatesAbb. 7-4: Micropla te with 96 wellsFig. 7-4: Microplate with

Table of contentsepMotion® 5070English (EN)46 Dispensing liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Labware – vessels, plates and pipette tipsepMotion® 5070English (EN)407.3 Reservoirs Reservoirs make liquid available. Reservoirs are available in 30

Labware – vessels, plates and pipette tipsepMotion® 5070English (EN)417.4 epT.I.P.S. Motion Abb. 7-8: epT.I.P.S. Motion 1 000 µL, 50µL, 300 µLFig.

Labware – vessels, plates and pipette tipsepMotion® 5070English (EN)427.4.1 epT.I.P.S. Motion RacksAbb. 7-9: epT.I.P.S. M otion RackFig. 7-9: epT.I.P

Labware – vessels, plates and pipette tipsepMotion® 5070English (EN)437.4.2 epT.I.P.S. Motion ReloadsepT.I.P.S. Motion Reloads are intended for single

Labware – vessels, plates and pipette tipsepMotion® 5070English (EN)447.4.3 Tip holder for epT.I.P.S. Motion ReloadsAbb. 7-12: Tip HolderFig. 7-12: Ti

Labware – vessels, plates and pipette tipsepMotion® 5070English (EN)457.4.4 epT.I.P.S. Motion SafeRacks Abb. 7-14: e pT.I.P.S. Motion SafeR ackFig. 7

Labware – vessels, plates and pipette tipsepMotion® 5070English (EN)46

Labware accessoriesepMotion® 5070English (EN)478 Labware accessories8.1 RacksRacks are holders for mounting the same type of individual vessels. 8.1.1

Labware accessoriesepMotion® 5070English (EN)488.1.2 Rack for 96 vesselsAbb. 8-2: Rack 96Fig. 8-2: Rack 96The Rack 96 takes up 2 locations on the wor

Labware accessoriesepMotion® 5070English (EN)498.1.3 Rack LCAbb. 8-3: Rack LC 100 µLFig. 8-3: Rack LC 100 µLThe Rack LC can hold 96 LightCycler capill

Table of contentsepMotion® 5070English (EN)59 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Labware accessoriesepMotion® 5070English (EN)508.1.5 Reservoir RackAbb. 8-6: Equipped Reservoir RackFig. 8-6: Equipped Reservoir RackThe Reservoir Rac

Labware accessoriesepMotion® 5070English (EN)518.1.6 Reservoir Rack modulesAbb. 8-7: Versions of Reservoir Rack modulesFig. 8-7: Versions of Reservoir

Labware accessoriesepMotion® 5070English (EN)528.1.6.2 Setting the Reservoir Rack module to vessel heightAbb. 8-9: Setting the Reservoir Rac k module

Labware accessoriesepMotion® 5070English (EN)53Tab. 8-1: Temperature control duration of Reservoir Rack modules and reaction vesselsThe table shows th

Labware accessoriesepMotion® 5070English (EN)548.2 Adapters The following adapters are available for the epMotion:• Height adapter•Thermoadapter8.2.1

Labware accessoriesepMotion® 5070English (EN)558.2.2 ThermoadapterAbb. 8-11: Thermoadapter DWP 96Fig. 8-11: Thermoadapter DWP 96Thermoadapters are ada

Labware accessoriesepMotion® 5070English (EN)568.2.4 Thermoadapter Frosty Abb. 8-13: Thermoadapter FrostyFig. 8-13: Thermoadapter FrostyThe Thermoadap

Labware accessoriesepMotion® 5070English (EN)578.3 Thermoblocks and Thermoadapters for PCR platesA thermoblock consists of a thermoadapter and a remov

Labware accessoriesepMotion® 5070English (EN)588.4 Waste container 8.4.1 Waste containerAbb. 8-18: W aste contain erFig. 8-18: Waste containerThe wast

Labware accessoriesepMotion® 5070English (EN)598.4.2 LiquidWasteTub with lid Abb. 8-19: LiquidWasteTub with lidFig. 8-19: LiquidWasteTub with lidThe L

Table of contentsepMotion® 5070English (EN)613 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Labware accessoriesepMotion® 5070English (EN)608.4.2.1 Using the LiquidWasteTub1. Place the disposal bag on the edge of the waste container (see p. 58

OperationepMotion® 5070English (EN)619 Operation9.1 First stepsThis chapter describes how to operate the epMotion and how to execute applications.  I

OperationepMotion® 5070English (EN)629.1.2.3 Data exchangeYou need a USB storage medium in order to exchange and backup data.1. Connect the USB storag

OperationepMotion® 5070English (EN)639.2 Loading the worktableTo prepare the epMotion for an application, perform the following steps:• Display the lo

OperationepMotion® 5070English (EN)649.2.2 Positioning tools on the worktable9.2.2.1 Dispensing tools When the application is started, epMotion check

OperationepMotion® 5070English (EN)659.2.4 Positioning the waste container on the worktable 1. Place the disposal bag in the waste container (see p.

OperationepMotion® 5070English (EN)66Plates, racks, epT.I.P.S Motion racks and Thermoadapters3. If required, tension the spring clip holder by hand.He

OperationepMotion® 5070English (EN)679.3.2 Starting the applicationPrerequisites• Application is selected.• Worktable is equipped (see p. 63).1. Start

OperationepMotion® 5070English (EN)689.3.3.1 Stopping the application immediatelyTo stop the application immediately, e.g., at risk of collision, proc

OperationepMotion® 5070English (EN)699.3.3.4 Aborting the application An application cannot be continued after it has been aborted.Prerequisites• The

Operating instructionsepMotion® 5070English (EN)71 Operating instructions1.1 Using this manualYour epMotion operating manual consists of hardware inst

OperationepMotion® 5070English (EN)709.4.2 Switching off the epMotionProceed as follows.1. Exit epBlue (refer to epMotion software operating manual).T

MaintenanceepMotion® 5070English (EN)7110 Maintenance10.1 Decontamination and cleaningThe device is cleaned as described in the Decontamination sectio

MaintenanceepMotion® 5070English (EN)7210.1.1 Carrying out wipe decontaminationPrerequisites• Decontamination agent• Deionized water• Lint-free cloths

MaintenanceepMotion® 5070English (EN)73Abb. 10-1: Removing the sing le-channel dispensing tool ejectorFig. 10-1: Removing the single-channel dispensin

MaintenanceepMotion® 5070English (EN)7410.1.3 AutoclavingPrerequisites• The temperature in the autoclave is 121 °C.• 100 kPa overpressure is present i

MaintenanceepMotion® 5070English (EN)7510.4 FusesFuses may only be replaced by authorized service technicians. Users must not replace the fuses.10.5 C

MaintenanceepMotion® 5070English (EN)768. Check to see if the dispensing tool dispenses liquid without errors by comparing the calculated volume of wa

MaintenanceepMotion® 5070English (EN)77Abb. 10-2: Exchanging the sealing ringsFig. 10-2: Exchanging the sealing ringsExchange the sealing rings as fol

MaintenanceepMotion® 5070English (EN)78

TroubleshootingepMotion® 5070English (EN)7911 TroubleshootingIf you cannot remedy an error with the recommended measures, please contact your local Ep

Operating instructionsepMotion® 5070English (EN)81.3 Symbols used1.4 Abbreviations usedDNADeoxyribonucleic acid (DNA)DWPDeepwell plateepT.I.P.S.eppend

TroubleshootingepMotion® 5070English (EN)8011.2 General errors11.2.1 Optical sensor errors Symptom/message Cause RemedyThe optical sensor does not det

TroubleshootingepMotion® 5070English (EN)8111.2.2 Dispensing errors 11.2.3 Software errorsThe software operating manual contains a description of soft

TroubleshootingepMotion® 5070English (EN)82

Transport, storage and disposalepMotion® 5070English (EN)8312 Transport, storage and disposal12.1 Transport Use the original packaging and the transp

Transport, storage and disposalepMotion® 5070English (EN)8412.3 DisposalIn case the product is to be disposed of, the relevant legal regulations are t

Technical dataepMotion® 5070English (EN)8513 Technical data13.1 Power supply13.2 Ambient conditions13.3 Weight/dimensions13.4 Noise level 13.5 Interfa

Technical dataepMotion® 5070English (EN)8613.6 EasyConOnly devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connec

Technical dataepMotion® 5070English (EN)8713.8 Dispensing tool errors13.8.1 PipettingDispensing tool Volume rangePipettingTesting volumeError limitsEr

Technical dataepMotion® 5070English (EN)8813.8.2 Dispensing13.9 Test conditions for dispensing tool errorsTest conditions and test evaluation in accor

Ordering InformationepMotion® 5070English (EN)8914 Ordering Information14.1 Tools14.2 epT.I.P.S. MotionOrder no. (International)DescriptionSingle-chan

Operating instructionsepMotion® 5070English (EN)91.5 GlossaryAApplicationPrograms for a specific application. An application includes the procedure an

Ordering InformationepMotion® 5070English (EN)90epT.I.P.S. Motion Filter 1 000 µL10 racks with 96 tips each0030 014.499 PCR clean0030 015.258 PCR clea

Ordering InformationepMotion® 5070English (EN)9114.3 Plates 14.4 ReservoirsAll twin.tec plates can be obtained with bar coding on request.Information

Ordering InformationepMotion® 5070English (EN)9214.5 RacksOrder no. (International)DescriptionRacks for individual tubesfor use with Eppendorf vessels

Ordering InformationepMotion® 5070English (EN)9314.6 Adapters14.7 ThermoblocksRack LC 5075 795.000 20 µL/100 µLThermorack CB5075 767.031 100 µLReservo

Ordering InformationepMotion® 5070English (EN)9414.8 AccessoriesWorktable base adapters may only be mounted by authorized service technicians.Order no

IndexepMotion® 5070English (EN)95IndexAAccessoriesOrdering information ...94AdaptersOrdering information ...

IndexepMotion® 5070English (EN)96MMTP ...8NNoise level ...

EG-Konformitätserklärung EC Conformity Declaration Das bezeichnete Produkt entspricht den einschlägigen grundlegenden Anforderungen der aufgeführten E