53
Centrifuge 5804/5804 R/5810/5810 R — Operating manual
Swing-bucket rotor
Prerequisites
• A combination of rotor, carrier and adapter, approved by Eppendorf.
• The carriers are sorted by weight category. Carriers located opposite each other must belong
to the same weight category. It is stamped onto the side of the groove, e.g. 68 (the last 2
digits in grams). When reordering - also plate carriers - make sure to specify the existing
weight category.
• Matching and tested tubes and plates.
• Do not remove the middle guiding elements of the modular adapters of the rectangular
buckets in order, e.g., to increase capacity through multi-level centrifugation.
To load the rotor, proceed as follows:
1. Check the carrier grooves for cleanliness and grease lightly with pivot grease (order
no.Int.:5810350.050/North America:022634330).
Dirty grooves and pivots prevent carriers from swinging out evenly.
2. Hang the buckets into the rotor.
All rotor positions must be loaded with carriers.
3. Check that all carriers are hanging properly and can swing freely.
Ensure that everything can swing freely
4. To check whether bottles, plates or tubes can swing freely, swing buckets manually.
Check swinging direction
5. To check whether the buckets including their load swing with the floor in the direction of the
rotor chamber wall, start turning the rotor cross anti-clockwise.
6. Check the maximum load per carrier (adapter, tube or plate and contents) and the loading
height.
The information about this can be found on the rotor and in this operating manual (see Rotors
on p. 13).
7. Load the buckets symmetrically.
Abb. 4: Incomplete, but symmetric loading of the buckets. The pegs of each bucket must be uniformly loaded.
Fig. 4: Incomplete, but symmetric loading of the buckets. The pegs of each bucket must be
uniformly loaded.
NOTICE!
Damage to adapters due to incorrect stacking.
Stack the adapters in rectangular buckets in a closed row only from the bottom of the bucket.
Do not leave any gaps between the modules.
NOTICE!
Filling the plates too high can cause overflowing.
During the run the meniscuses in the tubes along the edges of the plates are at an angle. This is
due to the centrifugal forces and cannot be avoided.
Fill the wells of the plates to a maximum of 2/3 of the max. capacity.
NOTICE!
Broken glass due to incorrect loading
Adapter 16x75 - 100mm: when the outer bore ring is loaded with tubes >119mm in length, there
is the risk of glass breakages.
Load the centre bore and the inner bore circle only.
EN
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