Eppendorf BioSpectrometer basic User Manual Page 54
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Methods
Eppendorf BioSpectrometer
®
basic
English (EN)
54
Peak detection
Press the [Peaks] softkey. For the peak detection you can alternate between two criteria:
• λ grid: Evaluation grid on the wavelength scale for the peak detection (e.g., 10 nm).
10 nm example: The spectra section from -5 nm to +5 nm is evaluated in relation to the peak to be
detected.
• Min. Δ Abs: Minimum difference between the peak to be detected and the lowest absorbance in the
evaluation grid. No absorbance value in the grid may be higher than the value of the peak at any given
time (e.g.: 0.5).
Examples:
λ grid: 100 nm, min. Δ Abs: 0.050:
The peak is not detected because the λ grid is too
large: The absorbance values on the left edge of the
grid are higher than the absorbance of the peak.
λ grid: 20 nm, min. Δ Abs: 0.200:
The peak is not detected because the predetermined
value for min. Δ abs is too high. The difference of the
absorbance of the peak and the lowest absorbance in
the grid is less than 0.2 A.
λ grid: 20 nm, min. Δ Abs: 0.050:
The peak is detected.
- Eppendorf BioSpectrometer 1
- Table of contents 3
- 1 Operating instructions 7
- 1.1 Using this manual 7
- 1.3 Symbols used 8
- 1.4 Abbreviations used 9
- Operating instructions 10
- English (EN) 10
- 2 Product description 11
- 2.1 Main illustration 11
- 2.2 Delivery package 11
- 2.3 Features 12
- 3 Safety 13
- 3.1 Intended use 13
- 3.2 User profile 13
- 3.3 Warnings for intended use 13
- 3.3.2 Damage to device 14
- Do not open the device 16
- 4 Installation 17
- 4.1 Preparing installation 17
- 4.2 Selecting the location 17
- 4.4 Connecting the printer 17
- 4.4.2 Thermal printer DPU-414 18
- Installation 20
- 5 Operation 21
- 5.1.1 Entering text 23
- 5.2 Inserting the cuvette 24
- 5.3.2 Measuring procedure 25
- 5.3.2.1 Selecting a method 25
- 5.3.2.2 Checking parameters 26
- 5.3.2.4 Measuring samples 27
- 5.3.2.5 Finalizing the method 27
- Operation 30
- 6 Methods 31
- 6.1 Selecting a method 31
- 6.2.1 Absorbance method group 32
- 6.2.2 Routine method group 33
- 6.2.3 Basic method group 34
- 6.3 Method parameters 35
- 6.4 Method procedure 40
- 6.4.1 Check parameters 41
- 6.4.2 Measure standards 42
- 6.4.3 Measure samples 43
- 6.4.5 Process results 50
- 6.4.7 Print & export 55
- 7 Functions 59
- 7.1.1 Results memory 60
- Confirm with enter 61
- 7.1.4 Device settings 64
- 7.1.5 Device calibration 64
- 7.1.6 Info 65
- Functions 66
- 8 Maintenance 67
- 8.1 Cleaning 67
- 8.3 Checking the device 69
- Maintenance 70
- 8.4 Replacing fuses 73
- 9 Troubleshooting 75
- 9.1 General errors 75
- 9.2 Error messages 76
- 9.3 Result flags 79
- Troubleshooting 82
- 10.1 Transport 83
- 10.2 Storage 83
- 10.3 Disposal 83
- 11 Technical data 85
- 11.1 Power supply 85
- 11.2 Ambient conditions 85
- 11.3 Weight/dimensions 85
- 11.4 Photometric properties 86
- 11.6 Application parameters 87
- Technical data 88
- 12 Evaluation procedure 89
- 12.1 Absorbance values 89
- 12.1.3 Cuvette correction 90
- 12.4 Dilution 92
- YYYXXXcorrXXX 93
- ACFAA u 93
- S, total 94
- 12.6.2 Calculation of the FOI 96
- 12.7 Dual wavelength 97
- Evaluation procedure 98
- 13 Ordering information 99
- Ordering information 100
- Evaluate your manual 104
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