Eppendorf BioSpectrometer basic User Manual Page 48
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Methods
Eppendorf BioSpectrometer
®
basic
English (EN)
48
Dye labels Results display:
• Concentration results with
absorbance at the measuring
wavelength of the biomolecule.
• If activated in the parameters:
Scan.
Navigate between the measuring
points in the graph with and .
Additional data ([Data] softkey):.
If the corresponding parameters have
been activated:
• Ratios A260/A280 and A260/A230.
• Absorbance values for 280 nm and
230 nm and for the measuring
wavelength of the dye.
• FOI value.
• Absorbance values for the
background wavelengths.
Ratios and FOI are not displayed for
the measurement of the dye-labeled
proteins.
Bacterial density Results display:
• Calculated result with absorbance
at the measuring wavelength.
• If activated in the parameters:
Scan.
Navigate between the measuring
points in the graph with and .
Basic main group
Factor, standard Analog to Protein direct UV (see above) Results display:
• Concentration result with
absorbance at the measuring
wavelength.
Calibration curve Analog to Proteins (with reagent) (see above) Results display:
• Concentration result with
absorbance at the measuring
wavelength.
• Graph of the standard evaluation
with plotted sample result.
Method group Results display Explanation
- Eppendorf BioSpectrometer 1
- Table of contents 3
- 1 Operating instructions 7
- 1.1 Using this manual 7
- 1.3 Symbols used 8
- 1.4 Abbreviations used 9
- Operating instructions 10
- English (EN) 10
- 2 Product description 11
- 2.1 Main illustration 11
- 2.2 Delivery package 11
- 2.3 Features 12
- 3 Safety 13
- 3.1 Intended use 13
- 3.2 User profile 13
- 3.3 Warnings for intended use 13
- 3.3.2 Damage to device 14
- Do not open the device 16
- 4 Installation 17
- 4.1 Preparing installation 17
- 4.2 Selecting the location 17
- 4.4 Connecting the printer 17
- 4.4.2 Thermal printer DPU-414 18
- Installation 20
- 5 Operation 21
- 5.1.1 Entering text 23
- 5.2 Inserting the cuvette 24
- 5.3.2 Measuring procedure 25
- 5.3.2.1 Selecting a method 25
- 5.3.2.2 Checking parameters 26
- 5.3.2.4 Measuring samples 27
- 5.3.2.5 Finalizing the method 27
- Operation 30
- 6 Methods 31
- 6.1 Selecting a method 31
- 6.2.1 Absorbance method group 32
- 6.2.2 Routine method group 33
- 6.2.3 Basic method group 34
- 6.3 Method parameters 35
- 6.4 Method procedure 40
- 6.4.1 Check parameters 41
- 6.4.2 Measure standards 42
- 6.4.3 Measure samples 43
- 6.4.5 Process results 50
- 6.4.7 Print & export 55
- 7 Functions 59
- 7.1.1 Results memory 60
- Confirm with enter 61
- 7.1.4 Device settings 64
- 7.1.5 Device calibration 64
- 7.1.6 Info 65
- Functions 66
- 8 Maintenance 67
- 8.1 Cleaning 67
- 8.3 Checking the device 69
- Maintenance 70
- 8.4 Replacing fuses 73
- 9 Troubleshooting 75
- 9.1 General errors 75
- 9.2 Error messages 76
- 9.3 Result flags 79
- Troubleshooting 82
- 10.1 Transport 83
- 10.2 Storage 83
- 10.3 Disposal 83
- 11 Technical data 85
- 11.1 Power supply 85
- 11.2 Ambient conditions 85
- 11.3 Weight/dimensions 85
- 11.4 Photometric properties 86
- 11.6 Application parameters 87
- Technical data 88
- 12 Evaluation procedure 89
- 12.1 Absorbance values 89
- 12.1.3 Cuvette correction 90
- 12.4 Dilution 92
- YYYXXXcorrXXX 93
- ACFAA u 93
- S, total 94
- 12.6.2 Calculation of the FOI 96
- 12.7 Dual wavelength 97
- Evaluation procedure 98
- 13 Ordering information 99
- Ordering information 100
- Evaluate your manual 104
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