Eppendorf BioPhotometer User Manual

BioPhotometerBedienungsanleitungOperating ManualMode d'emploiIstruzioni d'impiegoManual de Instrucciones BP_Titel_d_e_f_i_sp.fm Seite 1 Mo

52 2 Technical data Operation Cuvette material: dsDNA, ssDNA, RNA, Oligo, Protein: Quartz glass or plastic(UVette ® from Eppendorf)OD 600, Bradfor

53 Before using the Biophotometer please familiarize yourself completely with the operating instructions. The following points must be followed exactl

54 4 Installation Delivery package – BioPhotometer– Mains cable for BioPhotometer– Operating manual, incl. short instructions– Seal for cuvette shaft

55 4 Installation 4.2 Printer Printer DPU 414 The Eppendorf Thermal Printer DPU 414 can be connected to the serial interface RS-232 C of the BioPho

56 4 Installation Other printers In addition to the DPU 414, it is also possible to connect other serial printers to the serial interface of the Bio

57 5 Operation 5.1 Keypad – To call up the "Double-stranded DNA" method.– To enter figure 7.– To call up the "Single-stranded DNA&q

58 5 Operation – To call up the "BCA" and "BCA micro" methods.– To switch between the "BCA" and "BCA micro"

59 5 Operation 5.2 Measuring nucleic acids This description is valid for the following methods:– dsDNA– ssDNA– RNA– Oligo Call up methodCalculation

60 5 Operation Measure sampleResults display As an indication of the purity of the nucleic acid sample which has been measured, the absorbance at 23

61 5 Operation 5.3 Direct photometric measurement of protein Display after entry of "140 µ L sample volume" and "300 base pairs&quo

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62 5 Operation Measuring procedure The following example shows the measuring procedure for the "Absorbance" calculation mode. For details

63 5 Operation 5.4 Measuring proteins with reagent (Bradford, BCA, Lowry) Call up method If a valid calibration (which is then stored by the device

64 5 Operation Measuring procedure Blank measurements remain stored until the date changes(For further details, see "Measuring nucleic acids&qu

655 OperationDevice display following all standard measurements:The CV (coefficient of variation) is a measure of the scattering of standard values ar

665 Operation5.5 Measuring OD 600Select methodMeasuring procedureBlank measurements remain stored until the date changes (for further details, see &

675 Operation5.6 Measuring diluted samplesSample dilutions may be entered using the key before the measurement begins. When the result is calculat

685 Operation5.7 Changing the sample numberDuring sample measurements, the serial number of the sample appears in the top right of the display.The s

69 6 Programming 6.1 Programming procedure For each method, parameters such as the type of calculation or the unit of concentration are stored. The

70 6 Programming Example: Changing the unit Selection parameters are selected using the cursor keys and confirmed by pressing . The stored setting i

71 6 Programming 6.2 Overview of parameters (For "Factor" calculation only:)(For "Standard" calculation only:) Point 1: No sel

Nachdruck und Vervielfältigung – auch auszugsweise – nur mit Genehmigung.No part of this publication may be reproduced without the prior permission of

72 6 Programming 6.3 Explanation of parameters Parameters are defined as selection parameters or as parameters for entering numbers. In the case of

73 6 Programming The following parameters are offered only when the "Standard" calculation procedure has been programmed: Parameter Entrie

74 6 Programming 6.4 Factory-set programmed values Notes: 1) With"Factor" calculation: Input required from user2) With "Standard&quo

75 7 Functions Functions listFunction Entries Explanation Display results Call up using . Display of the last 100 results(The most-recent result app

76 7 Functions Example: Changing the language versionCall up functions listSelect desired functionStore function To exit the function level, either

77 8 Error messages, result flagging and help texts Result flaggingError texts in the results display Flagging Explanation 1.586 A 260 Flagging of A 2

78 8 Error messages, result flagging and help texts Error texts in measuring procedureError texts in calibration procedure Error text Explanation /

79 8 Error messages, result flagging and help texts Error texts in programming procedureOther error textsHelp texts CV greater than 10 % (Following s

80 Photometer – Disconnect the device from the main power source before carrying out maintenance work or to change the fuses.The inside of the device

81 10 Short instructions Preparation The BioPhotometer is ready to measure immediately after being switched on. Methods dsDNA ssDNA RNA

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82 10 Short instructions Programming The factory-set method programs may be changed as required. Example: Programming of the unit " µ g/mL"

83 10 Short instructions Measuring procedure for dsDNAdsDNAPROGRAMMED FACTOR 1 A260 = 50.0 µ g/mLSampledsDNA BLANKBlankdsDNA SAMPLE 001 20+200

84 10 Short instructions Bradford measuring procedureBRADFORDCALIBRATION RANGE100 – 2000 µ g/mLStandardBRADFORD BLANKBlank0 . 0 0 0 ABRADFORD SAMP

85 11.1 Ordering information Order no.Photometer 6131 000.012 BioPhotometer(230 V; 50/60 Hz; European plug)(additional power supply variants available

86 12 Calculation 12.1 Nucleic acids (dsDNA, ssDNA, RNA, oligo) Calculation via factor C= A 260 x FC = Calculated concentrationA 260 = Absorbanc

87 12 Calculation Conversion key: Calculating the molar concentration Application: Calculating the molar concentration from the mass concentration a

88 12 Calculation Sample dilution, optical light path of the cuvette and correction A 320 See Section 12.1. 12.3 Protein with addition of reagent

89 12 Calculation Sample dilution and optical light path of the cuvette See Section 12.1. 12.4 OD 600 The measured values appear as absorbance val

90 13 Testing the photometer To enable the photometric accuracy and the wavelength accuracy to be tested, a filter set (secondary UV-VIS filter) is ava

91 13 Testing the photometer Test procedure – Carry out the test at approximately 20 ° C.– Remove the filter from the filter box for a brief period o

47 1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

92 Conformity Declaration for BioPhotometer 6131 in accordance with enclosure 15 of "Eichordnung" (German standardization regulations)Descri

07.08.200389/336/EWG, EN 55011/B, EN 61000-6-1, EN 61000-3-2, EN 61000-3-373/23/EWG, EN 61010-1PhotometerBioPhotometer 61316131 900.994-01EG-Konformit

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Printed in Germany eppendorf ® is a registered trademark Your local distributor: www.eppendorf.com/worldwide Eppendorf AG22331 Hamburg · GermanyTel.

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49 1 Overview 1 Device display 5 Dilution key2 9 method keys 6 Conversion key3 Function key (device functions) 7 Measuring keys4 Parameter k

50 1 Overview Methods There are twelve preprogrammed factory-set methods which can be called up at the push of a button: Nucleic acidsdsDNA Double-s

51 2 Technical data Photometer Optical system: Absorption single-beam photometer with reference beamand several fixed wavelengthsIrradiation source: X