Eppendorf Research pro User Manual

®Eppendorf Research proInstruction Manual · Mode d’emploi · Manual de InstruccionesBA_Research_Titel_Rück_neu.fm Seite 1 Dienstag, 8. Juli 2008 6:

10Aspirating and dispensing speedsThe selected speed can be viewed by pressing the Speed key several times. The speed is changed as follows:* This pro

11In the Standard mode, blow-out is executed automatically when liquid is dispensed.The standard mode is recommended for rapid series pipetting with a

12BLO is recommended for use with liquids with a high wetting power or with liquids which are prone to the formation of foam (e.g. solutions containin

13Rinsing (RNS) is recommended for volumes below 10 µL. RNS is suitable for mixing the dispensing volume and the specimen liquid when large volumes ar

14RP is recommended for solutions with a high viscosity or with a slightly higher vapor pressure."Reverse Pipetting" is liquid aspiration wi

15Note: The MAN (manual) option is the equivalent of operation with a mechanical piston-stroke pipette. Liquid is aspirated and dispensed only when th

16Carrying out dispensing/titrationThe direction is changed again using the volume rocker. This change of direction can be repeated without restrictio

17FIX is used to rapidly set five frequently used pipetting volumes.Five FIX volumes are predefined in the pipette. FIX volumes can be changed via progr

18Selecting a fixed volume (with option and speed)Note: The option and speeds selected are applicable for all fixed volumes.It is possible to store the

19During this procedure, the aspirated liquid is dispensed in defined partial steps. As with "Reverse Pipetting", slightly more liquid is as

2Instruction Manual... 3Mode d’em

20 Free jet dispensing Free jet dispensing is possible at volumes greater than ≥ 20 µL. The small drop that forms after each free jet dispensing of

21To reach the programming level, the key must be double-clicked. PROG appears in the display together with a memory slot number from 1 – 5 .With

22 SDI = Serial Diluting For serial diluting, a defined volume of liquid is aspirated into the pipette tip. This liquid is dispensed and then mixed wi

23 Programming Programming can be ended prematurely and then stored by pressing the key. EDIT disappears from the display.If EDIT is ended with the

24 Device parameters may be changed only after thorough inspection and only by persons who are trained to do so! Device parameters are made up of the

25The Research pro must be calibrated only for solutions with a density, viscosity, surface tension and vapor pressure that are greatly different to t

26 Examples and notes for volume correction (balance in mg; density in mg/µL = g/mL).1. An actual volume of 98 µL was calculated using formula (1).

27The outside of the pipette can be wiped with a moist cloth. The use of water with cleaning wetting agent is also permitted.Do not allow any liquid t

28Assembly:– Hold down the ejector.– Position the lower part in such a way as to enable the metal pin on the ejector to enter the corresponding openin

29Apart from general care and occasional discharging (when the battery symbol starts flashing) and recharging of the pipette, no special maintenance is

3This operating manual is valid as of software version V.1.56 and data record 2.Software version and data record appear in the display after the batte

30 Display informationCause Solution Middle segment flashes. The battery is run down. Complete pipetting and recharge the battery (see Sec. 3.3). All

31 ErrorCause Solution Pipette is dripping; the volume dispensed is incorrect.– The tip may be loose.– There may be foreign matter between the pipett

32 If there is doubt that dispensing data are correct To avoid dispensing errors, the precision and accuracy of the Research pro need to be checked r

93 Programs... 93 I.

94This section contains a complete description of the additional seven program sequences (see Sec. 5.10).During programming ( EDIT and PROG in dis

95Select the memory slot.Selección del puesto de memoria. Hold down the key until EDIT appears. Mantener pulsada la tecla hasta que aparezca EDI

96 Procedure / Ejecución How you now proceed depends on the pipetting option selected and on the number of sequences. The procedure carried out acco

97 The dispensing procedure is described in Sec. 5.9.El procedimiento de dispensación se describe en el cap. 5.9. This program can be used when diffe

98Select the memory slot.Seleccionar el puesto de memoria. Hold down the key until EDIT appears. Mantener pulsada la tecla hasta que aparezca ED

99 Procedure / Ejecución The necessary or largest possible amount of liquid is aspirated. If appears in the display, press .Aspiración de la canti

4Intended useThe Research pro is a lab device intended for dispensing liquids in the volume range from 0.5 µL to 5 mL, in combination with matching pi

100When microtiter plates are filled, a specific amount of liquid must often be dispensed in rapid succession.The program is recommended for this task.

101 Procedure / Ejecución Select ADS .Seleccionar ADS .Select the dispensing volume, and store.Seleccionar el volumen de dispensación y memorizar.S

102 Changed procedure as of software version V.1.56!¡Procedimiento modificado en su ejecución a partir de la versión de software V. 1.56! Activation

103In this case, the reagent – or diluent – first and then the sample are aspirated into a pipette tip. Both liquids are initially separated by an air

104 Volume table Diluting (DIL); valid after data set 2Tabla de volúmenes Diluir (DIL); válida a partir del grupo de datos 2Procedure / Ejecución Sum

105In this program, pipetting is linked up to a specified number of mixing cycles. The program is used with a specimen liquid. The pipetting sample is

106 Procedure / Ejecución *1 Mixing volume is somewhat higher than displayed. *1 El volumen de mezcla es algo superior al indicado. Stroke for mixing

107From program version V.1.56 onwardsWith ASP, several volumes of the same size are aspirated simultaneously into a pipette tip. If the tip is filled

108 Procedure / Ejecución 1. Volume is aspirated.Se aspira el primer volumen.2. Volume is aspirated.Se aspira el segundo volumen.Step-by-step aspirat

109Eppendorf Research pro, single-channel / monocanalOrder no. / No de artículoincl. charging unit / con adaptador cargador * 0.5 – 10 µL 022461303inc

5The technical data is valid only when the Research pro is used with Eppendorf pipette tips. The multi-channel data is valid for eight- and twelve-cha

110Pipette tips / Puntas de pipeta – epT.I.P.S.The packing units stated represent the minimum order quantity.Las cantidades indicadas representan la

111Box, 1 box plus 96 tips / 1 caja más 96 puntas0.1 – 10 µL 34 mm dark gray* gris oscuro* 0224913000.1 – 20 µL 40 mm medium gray** gris** 0224913

112Singles (Eppendorf Biopur), individually wrapped, 1 set = 100 tipsSingles (Eppendorf Biopur), envasadas individualmente, 1 juego = 100 puntas0.1 –

113Racks, LoRetention PCR clean, 10 x 96 = 960 tipsRacks, LoRetention PCR clean, 10 x 96 = 960 puntas0.1 – 10 µL 34 mm dark gray gris oscuro 022493026

114Spare parts for single-channel pipettes / Piezas de repuesto para pipetas monocanalThe disassembly procedure can be found in Sec. 6.2 / Para el de

115Change Service parts (single-channel) / Cambio piezas de servicio (monocanal)The seals in the lower part (1) are unscrewed from the lower part (whi

116 Spare parts for multi-channel model / Piezas de repuesto para modelo multicanal The disassembly procedure can be found in Section 6.3 / Para el p

117 Change Service parts multi-channel up to 300 µLCambio piezas de servicio multicanal hasta 300 µL 1 Using the screwdriver, press in the catch and

118EC Conformity DeclarationEC Conformity DeclarationTeil 3 Bestellinfos E+S Seite 118 Donnerstag, 24. September 2009 3:17 15

Eppendorf OfficesEppendorf South Pacific Pty. Ltd.Tel. +61 2 98 89 50 00Fax +61 2 98 89 51 11E-Mail: [email protected]: www.eppendorf.com.au

6The Research pro is supplied as a single-channel, eight-channel or twelve-channel pipette for different volume ranges. The pipette is delivered with

In touch with lifeYour local distributor: www.eppendorf.com/worldwideEppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 538 01-0 · Fax: +49 40 538 0

74.1 Display and keypadOptionProgramSpeedRVolumeSelectEDIT1PROG 2345CALR50–1000 µLSpeedDisplay of speeds and by pressingkey repeatedly.Change by pres

8The Research pro is a microprocessor-controlled pipette which executes the piston movement with the aid of a stepper motor. The power supply is a rec

9The Research pro is switched on by pressing any key. Attach an Eppendorf pipette tip to the Research pro. The color coding of the Actuate key corresp