Eppendorf G0.5 µPlate User Manual

or useµPlate G0.5EN)ns for use Eppendorf® μPlate G0.5Instructions for use

OperationEppendorf® µPlate G0.5English (EN)105Operation5.1 Applications5.1.1 Nucleic acid quantificationFor the quantification procedure in the µPlate

OperationEppendorf® µPlate G0.5English (EN)115.2.3 Individual BlankingAll wells which will be used for the subsequent measurements will be included in

OperationEppendorf® µPlate G0.5English (EN)125.2.4 Sample ID functionThe user can use the "Sample ID" function to assign a unique name to ea

OperationEppendorf® µPlate G0.5English (EN)13Abb. 5-4:"Show Raw Data" checkboxFig. 5-4: "Show Raw Data" checkboxIf this box is sel

OperationEppendorf® µPlate G0.5English (EN)14Abb. 5-5:Raw data output in the Excel results summaryFig. 5-5: Raw data output in the Excel results summa

OperationEppendorf® µPlate G0.5English (EN)155.2.6 Editing sample typesIn the "Sample Type" dropdown menu, select the "Others” option.

OperationEppendorf® µPlate G0.5English (EN)165.2.7 Loading samples1. Open the µPlate G0.5.2. Pipette the samples into the wells.Abb. 5-7:µPlate G0.5 w

OperationEppendorf® µPlate G0.5English (EN)175.2.8 Loading samples using the pipetting aid1. Position the pipetting aid on the wells with the wells fa

OperationEppendorf® µPlate G0.5English (EN)185.3 Performing a measurement1. Start the PlateReader software.2. Connect the software to the PlateReader.

OperationEppendorf® µPlate G0.5English (EN)19An Excel worksheet opens automatically in the background while the measurement is running. All measuring

6144 900.209-01/062013 Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyr

OperationEppendorf® µPlate G0.5English (EN)20Abb. 5-11:Automatic summary of measuring resultsFig. 5-11: Automatic summary of measuring resultsIf all m

OperationEppendorf® µPlate G0.5English (EN)215.4 Quality control of the µPlate G0.55.4.1 Average blanking out of range (CV ≥ 10 %)If the coefficient o

OperationEppendorf® µPlate G0.5English (EN)225.4.2 Ending the blankingThere are two ways to end the blanking:1. Repeat the blanking using the same pla

TroubleshootingEppendorf® µPlate G0.5English (EN)236 Troubleshooting6.1 Working with the µPlate G0.5Always switch on the PlateReader AF2200 before loa

MaintenanceEppendorf® µPlate G0.5English (EN)247 Maintenance7.1 CleaningCleaning the µPlate G0.5 is particularly important in order to achieve optimal

Technical dataEppendorf® µPlate G0.5English (EN)258 Technical data8.1 Weight/dimensions8.2 Additional parametersWeightμPlate G0.5Weight approx. 160 gD

CalculationsEppendorf® µPlate G0.5English (EN)269 CalculationsWith each measurement, an additional measurement is performed automatically at a referen

CalculationsEppendorf® µPlate G0.5English (EN)279.2 Blank values9.2.1 Determining the average blank valueThe average absorbance value at 340 nm, which

CalculationsEppendorf® µPlate G0.5English (EN)289.3.2 Calculations based on an individual blank valueThe well-specific absorbance value at 340 nm is s

Ordering InformationEppendorf® µPlate G0.5English (EN)2910 Ordering InformationOrder no. (International)Order no. (North America)Description6144 000.0

Table of contentsEppendorf® µPlate G0.5English (EN)3Table of contents1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . .

TransportEppendorf® µPlate G0.5English (EN)3011 Transport11.1 Shipping the µPlate G0.5If you intend to ship the µPlate G0.5 to Eppendorf Service, obse

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Table of contentsEppendorf® µPlate G0.5English (EN)46 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Operating instructionsEppendorf® µPlate G0.5English (EN)51 Operating instructions1.1 Using this manual Read this operating manual completely before u

Product descriptionEppendorf® µPlate G0.5English (EN)62 Product description2.1 IntroductionThe µPlate G0.5 is a microvolume plate for quantifying smal

SafetyEppendorf® µPlate G0.5English (EN)73Safety3.1 Intended useThe µPlate G0.5 is designed for use in molecular biology, biochemistry and cell biolog

SafetyEppendorf® µPlate G0.5English (EN)83.3.2 Damage to deviceNOTICE! Damage from the use of aggressive chemicals. Do not use any aggressive chemica

InstallationEppendorf® µPlate G0.5English (EN)94 Installation4.1 Preparing installation Use the details included in the delivery package to check tha